Method of detecting a microorganism in a sample by a fluorescence based detection method
The present invention relates to a method of detecting a microorganism, in particular Staphylococcus aureus , in a sample, comprising the steps of a) incubating the sample with a slow off-rate modified aptamer (SOMAmer) comprising a fluorescent label, b) optionally washing the sample, c) analyzing the sample by a fluorescence-based detection method. The invention further relates to a slow off-rate modified aptamer comprising a fluorescent label, wherein the SOMAmer comprises a nucleotide sequence specific for Staphylococcus aureus , its use for detecting Staphylococcus aureus cells in a sample, and to a microarray or biosensor and a kit comprising at least one of such SOMAmers comprising a fluorescent label.
1. A method of detecting a microorganism in a sample, comprising
a) incubating the sample with a slow off-rate modified aptamer (SOMAmer) comprising a fluorescent label,
b) optionally washing the sample,
c) analyzing the sample by a fluorescence-based detection method,
wherein the SOMAmer comprises
the nucleotide sequence of SEQ ID NO:1, or
the nucleotide sequence which is fully complementary to SEQ ID NO:1,
wherein the microorganism is Staphylococcus aureus.
2. The method of claim 1 , wherein the fluorescence-based detection method is a method selected from the group consisting of flow cytometry, solid-phase cytometry, fluorescence-based analytical methods, and fluorescence imaging system.
3. The method of claim 1 , wherein the sample is a clinical sample, a food or feed sample, a beverage sample, a pharmaceutical sample, a personal care or cosmetic sample and/or a water sample.
4. The method of claim 1 , wherein the SOMAmer comprises a nucleotide sequence specific for Staphylococcus aureus.
5. The method of claim 1 , wherein the fluorescent label is selected from the group consisting of fluorescein dyes rhodamine dyes, cyanine dyes, Peridinin chlorophyll (PerCP), R-Phycoerythrin, coumarine dyes and quantum dots.
6. The method of claim 1 , wherein the SOMAmer comprises the nucleotide sequence of SEQ ID NO:1.