Lipid nanoparticle vaccine adjuvants and antigen delivery systems
The instant invention provides for novel lipid nanoparticle (LNP) formulations, containing cationic lipids, for use as vaccine adjuvants and/or as antigen delivery systems. It is an object of the instant invention to provide LNP formulations that demonstrate enhancements in humoral and cellular immunogenicity of vaccine antigens, particularly subunit vaccine antigens, when utilized alone or in combination with immunostimulatory agents (e.g. small molecule or oligonucleotide TLR agonists). The instant invention further identifies physical and chemical properties of the LNP formulations that can be manipulated to enhance antigen efficiency and adjuvant tolerability in vivo.
1. A method of enhancing an immune response in a subject comprising administering to the subject an effective amount of a composition comprising:
a) a lipid nanoparticle adjuvant (“LNP adjuvant”) comprising 34-59 mole % (13Z, 16Z)-N,N-dimethyl-3-nonyldocosa-13,16-dien-1-amine, 30-48 mole % cholesterol, 10-24 mole % distearoylphosphatidylcholine (DSPC) and 1-2 mole % polyethylene glycol-dimyristoylglycerol (PEG-DMG); and
b) one or more antigens;
wherein the composition provides an increased CD4+ T-cell response to the one or more antigens compared to a CD4+ T-cell response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
2. The method of claim 1 , wherein the composition further comprises an immunostimulatory agent selected from the group consisting of: saponin, squalene, aluminum phosphate and aluminum hydroxide.
3. The method of claim 1 , wherein the composition further comprises one or more agonists selected from the group consisting of: Toll-like receptors (TLR) agonists and Stimulator of Interferon Gene (STING) agonists.
4. The method of claim 1 , wherein the composition further provides an increased amount of antibody titers to the one or more antigens compared to an amount of antibody titers to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
5. The method of claim 1 , wherein the composition further provides an increased γ-interferon response the one or more antigens compared to a γ-interferon response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
6. The method of claim 1 , wherein the composition further provides an increased B-cell response to the one or more antigens compared to a B-cell response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
7. A method of enhancing an immune response in a subject comprising administering to the subject an effective amount of a composition comprising:
a) a lipid nanoparticle adjuvant (“LNP adjuvant”) comprising 34-59 mole % (13Z, 16Z)-N,N-dimethyl-3-nonyldocosa-13,16-dien-1-amine, 30-48 mole % cholesterol, 10-24 mole % distearoylphosphatidylcholine (DSPC) and 1-2 mole % polyethylene glycol-dimyristoylglycerol (PEG-DMG); and
b) one or more antigens;
wherein the composition provides an increased CD8+ T-cell response to the one or more antigens compared to a CD8+ T-cell response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
8. The method of claim 7 , wherein the composition further comprises an immunostimulatory agent selected from the group consisting of: saponin, squalene, aluminum phosphate and aluminum hydroxide.
9. The method of claim 7 , wherein the composition further comprises one or more agonists selected from the group consisting of: Toll-like receptors (TLR) agonists and Stimulator of Interferon Gene (STING) agonists.
10. The method of claim 7 , wherein the composition further provides an increased amount of antibody titers to the one or more antigens compared to an amount of antibody titers to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
11. The method of claim 7 , wherein the composition further provides an increased γ-interferon response the one or more antigens compared to a γ-interferon response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.
12. The method of claim 7 , wherein the composition further provides an increased B-cell response to the one or more antigens compared to a B-cell response to the one or more antigens provided by the composition in the absence of the LNP adjuvant.