DNA gridiron compositions and methods
View Patent ↗Novel compositions and methods for engineering wireframe architectures and scaffolds of increasing complexity by creating gridiron-like DNA structures (FIG. 1 ). A series of four-arm junctions are used as vertices within a network of double-helical DNA fragments. Deliberate distortion of the junctions from their most relaxed conformations ensures that a scaffold strand can traverse through individual vertices in multiple directions. DNA gridirons, ranging from two-dimensional arrays with reconfigurability to multilayer and three-dimensional structures and curved objects, can be assembled according the methods presented herein.
1. A composition, comprising:
a plurality of immobile Holliday junction analogs wherein each junction comprises a 60° torsion angle linked together in a square frame having at least two layers in which the helices on opposite sides lie in the same plane, and wherein said plurality of immobile Holliday junction analogs are linked together with a central strand of single-stranded DNA within said square frame.
2. The composition of claim 1 , wherein said plurality of immobile Holliday junction analogs are linked together in a square frame having at least three layers.
3. The composition of claim 1 , wherein said plurality of immobile Holliday junction analogs are linked together in a square frame having at least four layers.
4. The composition of claim 1 , wherein said single stranded DNA comprises M13mp18 DNA.