Glycosyl hesperetin and process for producing the same and uses thereof
The present invention aims to provide a novel glycosyl hesperetin, which is significantly reduced in miscellaneous tastes characteristic of conventional products containing glycosyl hesperetin, and a method for producing the same and uses thereof; and the objects are solved by providing a glycosyl hesperetin which comprises glycosyl hesperetin in an amount of 90% or more by mass but less than 100% by mass, on a dry solid basis, but it does not substantially contain furfural, and a method for producing the same and uses thereof.
1. A method for producing a glycosyl hesperetin composition, which comprises, as glycosyl hesperetin, α-glycosyl hesperidin and either or both of hesperidin and 7-O-β-glucosyl hesperetin in an amount of 90% or more by mass but less than 100% by mass, on a dry solid basis, wherein the content of furfural, measured on GC/MS analysis is less than 200 ppb, on a dry solid basis, the method comprising the steps of:
(a) providing an aqueous solution comprising hesperidin and a partial starch hydrolyzate;
(b) allowing a saccharide-transferring enzyme to act on the resulting aqueous solution to form glycosyl hesperetin; and
(c) collecting the formed glycosyl hesperetin to obtain the glycosyl hesperetin composition,
wherein one or more of the steps (a) to (c) are conducted in the presence of an inorganic reducing agent.
2. The method of claim 1 , wherein said saccharide-transferring enzyme used in the step (b) is one or more members selected from the group consisting of cyclomaltodextrin glucanotransferase, a-glucosidase, and a-amylase.
3. The method of claim 1 , wherein the enzymatic reaction solution obtained in the step (b) is subjected to the action of glucoamylase and/or a-L-rhamnosidase.
4. The method of claim 1 , wherein said inorganic reducing agent is a sulfite.