Devices and methods to improve and assess viability of human livers
The present invention relates to organ perfusion systems that can be used at room temperature. The organ perfusion systems do not comprise a temperature controller. In some embodiments, the organ perfusion systems do not comprise a cleaning device for cleaning the perfusion fluid. The perfusion fluid can comprise Williams' medium E. The organ perfusion systems can be portable and can be used to preserving an organ, preventing ischemic damage in an organ, or recovering an ischemically damaged organ.
1. An organ perfusion system operating at room temperature, the system comprising
an organ preservation container configured to encase an organ removed from a subject and store a perfusion fluid, whereby the organ is at least partially immersed in the perfusion fluid;
a fluidic circuit system having a first end connected to the perfusion fluid stored in the organ preservation container and a second end connected to the organ, the fluidic circuit system configured to draw the perfusion fluid through the first end and perfuse the organ with the perfusion fluid, and wherein the organ perfusion system does not comprise a temperature controller,
wherein the fluidic circuit system comprises one or more components,
wherein at least one of the one or more components is at least partially immersed in the perfusion fluid,
wherein the organ comprises a first blood vessel and a second blood vessel, and the fluidic circuit system comprises: (a) a first fluidic circuit connected to the first blood vessel and configured to flow a first perfusion fluid through the first blood vessel; and (b) a second fluidic circuit connected to the second blood vessel and configured to flow a second perfusion fluid through the second blood vessel, and
wherein the first fluidic circuit and the second fluidic circuit share a common fluidic pathway.
2. The organ perfusion system of claim 1 , wherein the organ perfusion system does not comprise a cleaning device for cleaning the perfusion fluid.
3. The organ perfusion system of claim 1 , wherein the organ perfusion system is portable.
4. The organ perfusion system of claim 1 , wherein the fluidic circuit system comprises a pressure sensor configured to measure a pressure of the perfusion fluid flowing towards the organ.
5. The organ perfusion system of claim 4 , wherein the fluidic circuit system comprises a pump configured to control a pressure of the perfusion fluid as a function of a measurement of the pressure sensor.
6. The organ perfusion system of claim 1 , wherein the fluidic circuit system comprises an oxygenator configured to increase oxygen level in the perfusion fluid flowing towards the organ.
7. The organ perfusion system of claim 1 , wherein the fluidic circuit system comprises a bubble-removing device configured to remove bubbles from the perfusion fluid flowing towards the organ.
8. The organ perfusion system of claim 1 , wherein the organ is selected from the group consisting of liver, pancreas, kidney, spleen, heart, lung, and a vascular composite tissue that can be cannulated for perfusion.
9. The organ perfusion system of claim 1 , wherein the perfusion fluid is a rich and defined medium.
10. The organ perfusion system of claim 1 , wherein the perfusion fluid comprises Williams' medium E.
11. The organ perfusion system of claim 1 , wherein the perfusion fluid further comprises insulin, one or more antibiotics, hydrocortisone, or any combinations thereof.
12. The organ perfusion system of claim 1 , wherein the perfusion fluid does not comprise red blood cells.
13. The organ perfusion system of claim 1 , further comprising a second container connected to the organ and configured to collect a product produced by the organ.
14. A method of preserving an organ, the method comprising connecting the organ to the organ perfusion system of claim 1 to perfuse the organ.
15. A method of preventing ischemic damage in an organ, the method comprising connecting the organ to the organ perfusion system of claim 1 to perfuse the organ.
16. The organ perfusion system of claim 1 , wherein the organ comprises a portal vein and an artery, and the fluidic circuit system comprises: (a) a first fluidic circuit connected to the portal vein and configured to flow a first perfusion fluid through the portal vein; and (b) a second fluidic circuit connected to the artery and configured to flow a second perfusion fluid through the artery, and wherein the first fluidic circuit and the second fluidic circuit share a common fluidic pathway.
17. The organ perfusion system of claim 16 , wherein the first fluidic circuit is independently controlled from the second fluidic circuit.
18. The organ perfusion system of claim 16 , wherein a pressure of the first perfusion fluid is in the range of 1-10 mmHg.
19. The organ perfusion system of claim 16 , wherein a pressure of the second perfusion fluid is in the range of 20-120 mmHg.
20. The organ perfusion system of claim 16 , wherein the first fluidic circuit and the second fluidic circuit share an oxygenator, a bubble-removing device, or a pressure sensor.