Implantable biocompatible reactor
The invention concerns a bioreactor obtained by compressing a mixture of an enzyme, a conductor and chitosan. The conductor can consist of carbon nanotubes. This bioreactor can be produced according to the following steps: preparing a mixture of powders in which the proportion of enzyme powder relative to a carbon nanotube powder is of the order of 50/50 by weight; preparing a viscous solution of chitosan in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% by volume in water; adding the viscous chitosan to the mixture of powders in a proportion be weight of 3 to 5 of powder to 5 to 10 of chitosan; carrying out a first compression followed by light grinding; carrying out a second compression to produce a pellet; and drying at ambient temperature.
1. A bioreactor obtained by a method comprising the steps of:
preparing a mixture of an enzyme powder and a carbon nanotube powder where the proportion of enzyme powder relative to carbon nanotube powder is of the order of 50/50 by weight;
preparing a viscous solution of chitosan in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% by volume in water;
adding the viscous chitosan to the mixture of enzyme powder and carbon nanotube powder in a proportion by weight of 3 to 5 of this mixture to 5 to 10 of chitosan;
carrying out a first compression followed by light grinding;
carrying out a second compression to produce a pellet; and
drying at ambient temperature.
2. The bioreactor of claim 1 , wherein the pressure applied during the first and the second compression is within a range from 2,000 to 6,000 kPa.
3. The bioreactor of claim 1 , wherein the solution comprises from 0.002 to 0.005% in mass per volume of genipin.
4. The bioreactor of claim 1 , wherein the solution comprises from 0.001 to 0.005% in mass per volume of caffeic acid.
5. The bioreactor of claim 1 , wherein a porous chitosan-based membrane is laid on an active surface of the bioreactor and bonded at the periphery thereof.
6. The bioreactor of claim 5 , wherein the porous chitosan-based membrane is obtained by a method comprising the steps of:
preparing a solution in a ratio of 5 to 15 (in mg) of chitosan to 0.75 to 1.25 (in ml) of acetic acid diluted to 0.4 to 0.6% in water;
stirring;
pouring on a smooth support; and
drying for a period from 2 to 4 days at ambient temperature.
7. The bioreactor of claim 5 , having a pellet-shaped bioelectrode, wherein a conductor is glued by means of conductive glue to the surface of the pellet-shaped bioelectrode opposite to the active surface.
8. The bioreactor of claim 5 , wherein the membrane comprises a plurality of pores having an average diameter in the range from 1 to 10 nanometers.
9. The bioreactor of claim 5 , wherein the membrane comprises a smooth surface facing the pellet-shaped electrode and a rough surface facing outwards.
10. The bioreactor of claim 7 , wherein the membrane comprises a plurality of pores having an average diameter in the range from 1 to 10 nanometers.
11. The bioreactor of claim 7 , wherein the membrane comprises a smooth surface facing the pellet-shaped electrode and a rough surface facing outwards.
12. The bioreactor of claim 2 , wherein the solution comprises from 0.002 to 0.005% in mass per volume of genipin.
13. The bioreactor of claim 2 , wherein the solution comprises from 0.001 to 0.005% in mass per volume of caffeic acid.