IP Library › Granted Patent US 10,494,625
Granted Patent B2
US 10,494,625 · App. 15/144,727 · Granted Dec 3, 2019

Hybrid polyketide synthases

Inventors: Jeffrey L. Fortman (San Francisco, CA); Andrew Hagen (Berkeley, CA); Leonard Katz (Oakland, CA); Jay D. Keasling (Berkeley, CA); Sean Poust (Berkeley, CA); Jingwei Zhang (San Francisco, CA); Sergey Zotchev (Tondheim, NO)
Assignee: The Regents of the University of California
C12N9/93C12N15/52C12P7/44C12P7/46C12P13/001C12P13/005C12P13/04C12P17/10C12P17/186C12Y604/00
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Quick Facts
Patent No.
US 10,494,625
App. No.
15/144,727
Granted
Dec 3, 2019
Kind
B2
Abstract

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

Claims (3)

1. A non-naturally occurring polyketide synthase (PKS) that binds malonyl-CoA and releases malonic acid, wherein said non-naturally occurring PKS is a hybrid PKS comprising domains from two PKSs, and comprises a single, three-domain chimeric PKS module comprising a didomain containing a malonyl-specific acyltransferase (AT) domain linked to a cognate acyl carrier protein (ACP) domain, and a type I thioesterase (TE) domain linked to said didomain;

wherein said didomain is from a load module from a PKS selected from the group consisting of a Streptomyces bikiniensis chalcomucin PKS, a Streptomyces caelestis niddamycin PKS, a Streptomyces avermitilis oligomycin PKS, and a Sorangium cellulosum epothilone PKS; and

wherein said TE domain is from a Streptomyces venezuelae pikromycin PKS or a Saccharopolyspora erythraea erythromycin PKS.

Assignments (2)
CONFIRMATORY LICENSE Recorded Jul 7, 2016
From: REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE
To: ENERGY, UNITED STATES DEPARTMENT OF
Reel/Frame 039303/0386 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 3, 2016
From: FORTMAN, JEFFREY L.; HAGEN, ANDREW; KATZ, LEONARD; KEASLING, JAY D.; POUST, SEAN; ZHANG, JINGWEI; ZOTCHEV, SERGEY
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 039068/0590 →
Continuity (3)
Continuation 13882099
Provisional Application 61408411 · Oct 29, 2010
Related Publication 20170015990A1 · Jan 19, 2017