Gene impacting biomass formation and recalcitrance and methods of use
The invention provides plants having improved biomass properties of increased biomass quantity and density, increased cellulose content and deceased lignin content and improved sugar release efficiency, methods of generating (genetically modifying) and/or selecting (natural variant collections) plants with such preferred attributes of cellulose, lignin and sugar release, and uses of such plants. The inventors have determined that the expression of the gene, Potri.001G375700 (belonging to IQD signaling protein family containing a canonical calmodulin-binding domain) and/or activity of Potri.001G375700, modulates cellulose, lignin synthesis, sugar release and growth in plants.
1. A method for increasing biomass, cellulose content or sugar release in a Populus plant, the method comprising:
(i) transforming Populus plants with a recombinant binary vector comprising a DNA expression construct, wherein the DNA expression construct comprises a heterologous promoter operably linked to a first DNA segment of 100-500 nucleotides long that corresponds to at least a portion of the 3′ UTR region of the IQD1 gene as set forth in SEQ ID NO: 1, a spacer segment and a second DNA segment that is fully complementary to the first DNA segment, wherein the first and second DNA segments are arranged in a 5′ to 3′ direction, respectively, in the DNA expression construct;
(ii) expressing said DNA construct to produce double stranded with hairpin loop RNAi inhibitory molecule in said transformed Populus plants, and wherein expression of endogenous IQD1 gene and its encoded IQD1 protein in said Populus plants is reduced;
and
(iii) selecting a transformed Populus plant from transformed Populus plants of step (ii) which expresses said RNAi inhibitory molecule and exhibits increase in biomass, cellulose content or sugar release as compared to a wild-type untransformed Populus plant species lacking said DNA expression construct.
2. The method according to claim 1 , wherein said biomass is increased in said selected transformed plant.
3. The method according to claim 1 , wherein said cellulose content is increased in said selected transformed plant.
4. The method according to claim 1 , wherein said sugar release is increased in said selected transformed plant.