Methods and systems for laser speckle imaging of tissue using a color image sensor
Methods and systems for imaging tissue of a subject are disclosed, and involve illuminating the tissue with a coherent light having a coherent wavelength, acquiring image data of the tissue using a color image sensor, and processing the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue. The perfusion image is then displayed to the user. Also disclosed are methods and systems for correcting for ambient light and for acquiring white light images along with laser speckle images.
1. A method for imaging tissue of a subject, the method comprising:
illuminating the tissue with a coherent light having a coherent wavelength;
acquiring image data of the tissue using a color image sensor, said color image sensor having pixels, some of the pixels corresponding to a first color, some of the pixels corresponding to a second color, and some of the pixels corresponding to a third color; and
processing the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein the correction includes applying a first correction factor to the first color pixels, a second correction factor to the second color pixels and a third correction factor to the third color pixels.
2. The method of claim 1 wherein the correction factors are determined during image data acquisition.
3. The method of claim 1 further comprising alternating the coherent light between a turned on state and a turned off state during image data acquisition.
4. The method of claim 3 wherein the image data acquired during the turned off state of the coherent light comprises white light image data to generate a white light image.
5. The method of claim 1 further comprising displaying the perfusion image.
6. The method of claim 1 wherein the coherent wavelength ranges from about 750 nm to about 850 nm.
7. A method for imaging tissue of a subject, the method comprising:
illuminating the tissue with a coherent light having a coherent wavelength;
acquiring image data of the tissue using a color image sensor; and
processing the image data using laser speckle contrast analysis while correcting for differences in sensitivity of different color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein correcting for difference in sensitivity of color pixels at the coherent wavelength comprises changing color channel dependent analog or digital gain of the image sensor.
8. A method for imaging tissue of a subject, the method comprising:
illuminating the tissue with a coherent light having a coherent wavelength;
acquiring image data of the tissue using a color image sensor; and
processing the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein correcting for differences in sensitivity of color pixels at the coherent wavelength comprises calculating separate speckle images based on each set of like color pixels, and combining the calculated speckle images to calculate the perfusion image.
9. A system for imaging tissue of a subject, the system comprising:
a coherent light source to generate coherent light having a coherent wavelength;
a color image sensor to acquire image data of the tissue, said color image sensor having pixels, some of the pixels corresponding to a first color, some of the pixels corresponding to a second color, and some of the pixels corresponding to a third color; and
a processor to process the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein the correction includes applying a first correction factor to the first color pixels, a second correction factor to the second color pixels and a third correction factor to the third color pixels.
10. The system of claim 9 wherein the correction factors are determined during manufacturing of the system, calibration of the system, use of the system, or a combination thereof.
11. The system of claim 9 further comprising a control unit in communication with the color image sensor, the processor, or a combination thereof to control the color image sensor, the processor, the coherent light source, or a combination thereof.
12. The system of claim 9 further comprising means to alternate the coherent light between a turned on state and a turned off state during image data acquisition.
13. The system of claim 12 wherein the image data acquired during the turned off state of the coherent light comprises white light image data to generate a white light image.
14. The system of claim 13 wherein the image data comprises ambient light image data, and the processor further generates an ambient light image.
15. The system of claim 13 further comprising a display to display the perfusion image and the white light image.
16. The system of claim 9 wherein the coherent wavelength ranges from about 750 nm to about 850 nm.
17. The system of claim 9 further comprising a display to display the perfusion image.
18. A system for imaging tissue of a subject, the system comprising:
a coherent light source to generate coherent light having a coherent wavelength;
a color image sensor to acquire image data of the tissue; and
a processor to process the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein correcting for difference in sensitivity of color pixels at the coherent wavelength comprises changing color channel dependent analog or digital gain of the image sensor.
19. A system for imaging tissue of a subject, the system comprising:
a coherent light source to generate coherent light having a coherent wavelength;
a color image sensor to acquire image data of the tissue; and
a processor to process the image data using laser speckle contrast analysis while correcting for differences in sensitivity of color pixels at the coherent wavelength to generate a perfusion image of the tissue wherein the correction for differences in sensitivity of color pixels at the coherent wavelength comprises a calculation of separate speckle images based on each set of like color pixels, and combining the calculated speckle images to calculate the perfusion image.