Optimized Fc variants and methods for their generation
The present invention relates to optimized Fc variants, methods for their generation, and antibodies and Fc fusions comprising optimized Fc variants.
1. A nucleic acid encoding a polypeptide comprising an Fc variant of a human IgG Fc polypeptide, said Fc variant comprising an amino acid substitution at position 328 in the Fc region, wherein numbering is according to the EU index.
2. The nucleic acid according to claim 1 , wherein the substitution at position 328 is selected from the group consisting of M, E, and F.
3. The nucleic acid according to claim 1 , wherein the substitution at position 328 is 328F.
4. The nucleic acid according to claim 1 , wherein the human IgG Fc polypeptide is a human IgG1 Fc polypeptide.
5. An expression vector comprising the nucleic acid according to claim 1 .
6. A host cell comprising a nucleic acid according to claim 1 .
7. A host cell comprising an expression vector according to claim 5 .
8. A method of producing a polypeptide comprising: a) culturing a host cell according to claim 6 under conditions wherein said polypeptide is produced; and b) purifying said polypeptide.
9. A method of producing a polypeptide comprising: a) culturing a host cell according to claim 7 under conditions wherein said polypeptide is produced; and b) purifying said polypeptide.
10. A nucleic acid encoding a heavy chain of an antibody comprising an Fc variant of a human IgG Fc polypeptide, said Fc variant comprising an amino acid substitution at position 328 in the Fc region, wherein numbering is according to the EU index.
11. The nucleic acid according to claim 10 , wherein the substitution at position 328 is selected from the group consisting of M, E, and F.
12. The nucleic acid according to claim 10 , wherein the substitution is 328F.
13. The nucleic acid according to claim 10 , wherein the human IgG Fc polypeptide is a human IgG1 Fc polypeptide.
14. The nucleic acid according to claim 10 , wherein the antibody is a monoclonal antibody.
15. An expression vector comprising the nucleic acid according to claim 10 .
16. A host cell comprising a nucleic acid according to claim 10 .
17. A host cell comprising an expression vector according to claim 15 .
18. A method of producing a polypeptide comprising: a) culturing a host cell according to claim 16 under conditions wherein said polypeptide is produced; and b) purifying said polypeptide.
19. A method of producing a polypeptide comprising: a) culturing a host cell according to claim 17 under conditions wherein said polypeptide is produced; and b) purifying said polypeptide.