IP Library › Granted Patent US 9,642,317
Granted Patent B2
US 9,642,317 · App. 15/152,253 · Granted May 9, 2017

Breeding, production, processing and use of specialty cannabis

Inventors: Mark Anthony Lewis (Los Angeles, CA); Michael D Backes (Los Angeles, CA); Matthew W Giese (Westlake Village, CA)
Assignee: Biotech Institute, LLC.
A01H5/02
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Quick Facts
Patent No.
US 9,642,317
App. No.
15/152,253
Granted
May 9, 2017
Kind
B2
Abstract

The invention provides compositions and methods for the breeding, production, processing and use of specialty cannabis.

Claims (49)

1. A cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescence comprising:

a) at least one B T allele, and at least one B D allele;

b) cannabidiol (CBD) cannabinoid content of at least 1.5% by weight;

c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than 1.4; and

d) a terpene oil content greater than about 1.0% by weight;

wherein the contents of THC and CBD comprise acidic and decarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein the terpene oil content is the additive content of the terpenes in the inflorescence's terpene profile; wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene; and wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and are calculated based on dry weight of the inflorescence; wherein a representative sample of seed capable of producing said plants has been deposited under NCIMB Nos. 42248, 42249, and wherein cellular cultures representative of said plants are deposited under Bigelow NCMA Nos. PATENT201611001, PATENT201609001, and PATENT201612001.

2. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 wherein the inflorescence comprises a terpene profile in which myrcene is not the dominant terpene.

3. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the THC:CBD content ratio is greater than 1.5.

4. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the THC:CBD content ratio is greater than 1.6.

5. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the THC:CBD content ratio is greater than 1.7.

6. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the THC:CBD content ratio is greater than 1.8.

7. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the inflorescence comprises a CBD content that is at least 3.0% by weight.

8. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the inflorescence comprises a THC content that is at least 6.0% by weight.

9. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein the inflorescence comprises a CBD content that is at least 6.0% by weight.

10. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 2 , wherein limonene is the most abundant terpene in said terpene profile.

11. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B T allele, and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, and a terpene oil content greater than about 1.0% by weight, said method comprising:

(i) making a cross between a first cannabis plant of claim 1 , or an asexual clone of said first cannabis plant and a second cannabis plant to produce an F1 seed:

(ii) harvesting the resulting seed;

(iii) growing one or more of said harvested seed; and

(iv) selecting a cannabis plant with a desired phenotype;

wherein the resulting selected cannabis plant produces an inflorescence comprising at least one B T allele, and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, and a terpene oil content greater than about 1.0% by weight.

12. A method of producing a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B T allele, and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, and a terpene oil content greater than about 1.0% by weight, said method comprising:

(i) obtaining a cannabis seed, cutting, or plant cell, from a cannabis plant, or an asexual clone of said cannabis plant of claim 1 ;

(ii) placing said cannabis seed, cutting, or plant cell in an environment conducive to growth; and

(iii) allowing said cannabis seed, cutting, or plant cell to produce a cannabis plant;

wherein the produced cannabis plant produces an inflorescence comprising at least one B T allele, and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, and a terpene oil content greater than about 1.0% by weight.

13. A cannabis extract from the cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 .

14. The cannabis extract of claim 13 , wherein said extract is selected from the group consisting of kief, hashish, bubble hash, solvent reduced oils, sludges, e-juice, and tinctures.

15. An edible product comprising cannabis tissue from the cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 .

16. An edible product comprising the cannabis extract of claim 13 .

17. The cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof of claim 1 , wherein said plant part is selected from the group consisting of: trichomes, sun leaves, or inflorescences.

18. A progeny plant derived from the cross of SIL08×CBD05, said progeny plant being capable of producing a female inflorescence, said inflorescence comprising:

a) at least one B T allele, and at least one B D allele;

b) a cannabidiol (CBD) cannabinoid content of at least 1.5% by weight; and

c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than 1.4; and

wherein the contents of THC and CBD comprise acidic and decarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein seed of said progeny plants has been deposited under NCIMB No. 42248.

19. A cannabis plant, or an asexual clone of said cannabis plant, or a plant part, tissue, or cell thereof, which produces a female inflorescence, said inflorescence comprising:

a) at least one B T allele, and at least one B D allele;

b) cannabidiol (CBD) cannabinoid content of at least 1.5% by weight;

c) a tetrahydrocannabinol (THC) cannabinoid content such that the THC:CBD content ratio is greater than 1.4;

d) a terpene oil content greater than about 1.0% by weight; and

e) a terpene profile in which myrcene is not the dominant terpene;

wherein the contents of THC and CBD comprise acidic and decarboxylated cannabinoids as measured by high performance liquid chromatography (HPLC) and calculated based on dry weight of the inflorescence; wherein the terpene oil content is the additive content of the terpenes in the inflorescence's terpene profile; wherein the terpene profile consists of terpinolene, alpha phellandrene, beta ocimene, carene, limonene, gamma terpinene, alpha pinene, alpha terpinene, beta pinene, fenchol, camphene, alpha terpineol, alpha humulene, beta caryophyllene, linalool, caryophyllene oxide, and myrcene; and wherein the terpene contents are measured by gas chromatography-flame ionization detection (GC-FID) and are calculated based on dry weight of the inflorescence; wherein a representative sample of seed capable of producing said plants has been deposited under NCIMB No. 42249, and wherein cellular cultures representative of said plants are deposited under Bigelow NCMA Nos. PATENT201611001, and PATENT201609001.

20. A method of breeding for a cannabis plant, wherein said cannabis plant produces an inflorescence comprising at least one B T allele and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, a terpene oil content greater than about 1.0% by weight, and a terpene profile in which myrcene is not the dominant terpene, said method comprising:

(i) making a cross between a first cannabis plant of claim 19 , or an asexual clone of said first cannabis plant and a second cannabis plant to produce an F1 seed:

(ii) harvesting the resulting seed;

(iii) growing one or more of said harvested seed; and

(iv) selecting a cannabis plant with a desired phenotype;

wherein the resulting selected cannabis plant produces an inflorescence comprising at least one B T allele, and at least one B D allele, a CBD content of at least 1.5% by weight, a THC:CBD content ratio higher than 1.4, a terpene oil content greater than about 1.0% by weight and a terpene profile in which myrcene is not the dominant terpene.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 11, 2016
From: LEWIS, MARK ANTHONY; BACKES, MICHAEL D; GIESE, MATTHEW W.
To: BIOTECH INSTITUTE, LLC
Reel/Frame 038552/0459 →
Continuity (5)
Continuation 14742493 · Jun 17, 2015
Continuation 14216744 · Mar 17, 2014
Provisional Application 61801528 · Mar 15, 2013
Provisional Application 61897074 · Oct 29, 2013
Related Publication 20160324091A1 · Nov 10, 2016