Diagnostic method for urinary tract infection
It has been demonstrated that the urinary level of HBP increases in individuals that have a urinary tract infection. Accordingly, the urinary level of HBP in an individual can be used to determine whether or not an individual has a urinary tract infection.
1. A method of treating an individual having a urinary tract infection (UTI), comprising:
measuring the level of Heparin Binding Protein (HBP, CAP37, Azuricidin) in a urine sample obtained from an individual by;
(i) contacting the sample with an antibody which binds specifically to HBP; and
(ii) quantitating the amount of antibody-HBP complex to measure the HBP level in the urine sample;
detecting the presence of UTI in the individual at about 93% Sensitivity or greater and at about 90% Specificity or greater based on the measured HBP level in the urine sample; and
administering to the individual detected as having UTI an effective amount a therapeutic agent suitable for treating UTI.
2. The method of claim 1 , wherein the individual is a mammal.
3. The method of claim 2 , wherein the mammal is a human.
4. The method of claim 3 , wherein the detecting of UTI at about 93% Sensitivity or greater and at about 90% Specificity or greater is determined by a Receiver Operating Characteristic (ROC).
5. The method of claim 3 , wherein the 93% Sensitivity and the 90% Specificity for detecting UTI is a threshold value of about 32 ng/ml in the urine sample.
6. The method of claim 1 , wherein the binding of the antibody to HBP is measured by enzyme linked immunosorbent assay (ELISA).
7. The method of claim 1 , wherein the binding of the antibody to HBP is measured using a dipstick assay.
8. The method of claim 1 , wherein the urinary tract infection is lower urinary tract infection or pyelonephritis.
9. The method of claim 1 , further comprising determining white blood cell count or level of IL6 in a urine sample from the individual, or conducting a nitrite test on a urine sample from the individual.