Non R-gene mediated resistance
The invention relates to plants and plant parts, in particular spinach plants ( Spinacia oleracea L.), which are resistant to Peronospora farinosa f. sp. spinaciae . The invention also relates to seeds capable of producing Peronospora farinosa f. sp. spinaciae resistant plants. The invention further relates to methods for obtaining said plants with altered genotypes and seeds thereof, which are resistant to Peronospora farinosa f. sp. spinaciae.
1. A method of selecting a Spinacia oleracea plant comprising the p10 locus of seed deposited with the NCIMB under accession number 42554 and showing at least intermediate resistance to Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:7, Pfs:8, Pfs:9, Pfs:10, Pfs 11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and Pfs:16 that is conferred by the downy mildew resistance contributing locus p10, the method comprising detecting in a Spinacia oleracea plant a marker having SEQ ID No. 1 and a marker having SEQ ID No. 3, and selecting a Spinacia oleracea plant that comprises said the marker having SEQ ID No. 1 and the marker having SEQ ID No. 3 as the Spinacia oleracea plant comprising the p10 locus.
2. The method of claim 1 , including performing one or more phenotypic assays for Peronospora farinosa f. sp. spinaciae resistance to select a Spinacia oleracea plant having resistance to Peronospora farinosa f. sp. spinaciae.
3. A method of producing an agronomically elite hybrid Spinacia oleracea seed comprising
crossing a first parent Spinacia oleracea plant with a second parent Spinacia oleracea plant and
harvesting the resultant hybrid Spinacia oleracea seed, wherein said first parent Spinacia oleracea plant and/or said second parent Spinacia oleracea plant comprises
(a) a plant grown from seed deposited with the NCIMB under accession number 42554, or
(b) progeny thereof having the p10 locus located on chromosome 1 and showing a pattern of monogenic recessive inheritance of the seed deposited with the NCIMB under accession number 42554 and at least intermediate resistant resistance to Peronospora farinosa f. sp. spinaciae races Pfs:1, Pfs:2, Pfs:3, Pfs:4, Pfs:5, Pfs:6, Pfs:7, Pfs:8, Pfs:9, Pfs:10, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15 and Pfs:16 that is conferred by the downy mildew resistance contributing locus p 10, and comprising the marker having SEQ ID No. 1 and the marker having SEQ ID No. 3, or
(c) a plant selected as comprising the p10 locus by the method of claim 1 or 2 .
4. A method of determining the genotype of a Spinacia oleracea plant comprising the p 10 locus of seed deposited with the NCIMB under accession number 42554 comprising detecting in the Spinacia oleracea plant a marker having SEQ ID No. 1 and a marker having SEQ ID No. 3.
5. A method for producing an agronomically elite Spinacia oleracea plant comprising at least intermediate resistance to Peronospora farinosa f. sp. spinaciae Pfs: 1-16, comprising:
(a) crossing a first plant selected as comprising the p10 locus by the method of claim 1 or 2 or a plant determined to have the genotype of a Spinacia oleracea plant comprising the p10 locus by the method of claim 4 , with a second plant; and
(b) performing one or more rounds of selfing and/or crossing.
6. The method of claim 5 , further comprising: (c) selecting after each round of selfing or crossing for a plant that comprises said resistance.