Nucleic acids encodng anti-C5A antibodies
The present disclosure relates to, inter alia, antibodies, or antigen-binding fragments thereof, that bind to C5a and to use of the antibodies in methods for treating or preventing complement-associated disorders such as, but not limited to, atypical hemolytic uremic syndrome, age-related macular degeneration, rheumatoid arthritis, sepsis, severe burn, antiphospho lipid syndrome, asthma, lupus nephritis, Goodpasture's syndrome, and chronic obstructive pulmonary disease.
1. A nucleic acid encoding complementarity determining regions (CDRs) of a heavy chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human C5a, wherein the heavy chain variable region comprises the amino acid sequence depicted in SEQ ID NO:45.
2. A nucleic acid encoding complementarity determining regions (CDRs) of a light chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human C5a, wherein the light chain variable region comprises the amino acid sequence depicted in SEQ ID NO:42.
3. A nucleic acid encoding complementarity determining regions (CDRs) of heavy and light chain variable regions of an antibody, or antigen-binding fragments thereof, that bind to human C5a, wherein the heavy and light chain variable regions comprise the amino acid sequences depicted in SEQ ID NOs:45 and 42, respectively.
4. A nucleic acid encoding a heavy chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human C5a, wherein the heavy chain variable region comprises the amino acid sequence depicted in SEQ ID NO:45.
5. A nucleic acid encoding a light chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human C5a, wherein the light chain variable region comprising the amino acid sequence depicted in SEQ ID NO:42.
6. A nucleic acid encoding heavy and light chain variable regions of an antibody, or antigen-binding fragments thereof, that bind to human C5a, wherein the heavy and light chain variable regions comprise the amino acid sequences depicted in SEQ ID NOs:45 and 42, respectively.
7. A nucleic acid encoding a heavy chain of an antibody, or antigen-binding fragment thereof, that binds to C5a, wherein the heavy chain comprises the amino acid sequence depicted in SEQ ID NO: 49.
8. A nucleic acid encoding a light chain of an antibody, or antigen-binding fragment thereof, that binds to C5a, wherein the light chain comprises the amino acid sequence depicted in SEQ ID NO: 40.
9. A nucleic acid encoding a heavy chain and a light chain of an antibody, or antigen-binding fragment thereof, that binds to human C5a, wherein the heavy chain comprises the amino acid sequence depicted in SEQ ID NO: 49 and the light chain comprises the amino acid sequence depicted in SEQ ID NO: 40.
10. An expression vector comprising the nucleic acid according to claim 1 .
11. A cell comprising the expression vector according to claim 10 .
12. An expression vector comprising the nucleic acid according to claim 2 .
13. A cell comprising the expression vector according to claim 12 .
14. An expression vector comprising the nucleic acid according to claim 3 .
15. A cell comprising the expression vector according to claim 14 .
16. An expression vector comprising the nucleic acid according to claim 4 .
17. A cell comprising the expression vector according to claim 16 .
18. An expression vector comprising the nucleic acid according to claim 5 .
19. A cell comprising the expression vector according to claim 18 .
20. An expression vector comprising the nucleic acid according to claim 6 .
21. A cell comprising the expression vector according to claim 20 .
22. An expression vector comprising the nucleic acid according to claim 7 .
23. A cell comprising the expression vector according to claim 22 .
24. An expression vector comprising the nucleic acid according to claim 8 .
25. A cell comprising the expression vector according to claim 24 .
26. An expression vector comprising the nucleic acid according to claim 9 .
27. A cell comprising the expression vector according to claim 26 .