Antibodies reactive with B7-H3 and uses thereof
The present invention relates to antibodies that are immunoreactive to the mammalian, and more particularly, the human B7-H3 receptor and to uses thereof, particularly in the treatment of cancer and inflammation. The invention thus particularly concerns humanized B7-H3-reactive antibodies that are capable of mediating, and more preferably enhancing the activation of the immune system against cancer cells that are associated with a variety of human cancers.
1. A nucleic acid molecule comprising a sequence encoding a polypeptide comprising:
(A) a light chain having the amino acid sequence of SEQ ID NO: 117; or
(B) a heavy chain having the amino acid sequence of SEQ ID NO: 119.
2. The nucleic acid molecule of claim 1 , wherein the encoded polypeptide comprises the light chain having the amino acid sequence of SEQ ID NO: 117.
3. The nucleic acid molecule of claim 1 , wherein the encoded polypeptide comprises the heavy chain having the amino acid sequence of SEQ ID NO: 119.
4. The nucleic acid molecule of claim 3 , wherein the encoded polypeptide further comprises the light chain having the amino acid sequence of SEQ ID NO: 117.
5. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule comprises the light chain sequence of SEQ ID NO: 118.
6. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule comprises the heavy chain sequence of SEQ ID NO: 120.
7. The nucleic acid molecule of claim 6 , wherein said nucleic acid molecule further comprises the light chain sequence of SEQ ID NO: 118.
8. The nucleic acid molecule of claim 1 , wherein the encoded polypeptide is a single chain antibody, a monoclonal antibody, or a diabody.
9. A vector comprising the nucleic acid molecule of claim 1 .
10. The vector of claim 9 , which is an expression vector.
11. A host cell comprising the expression vector of claim 10 .
12. A method of producing a substantially purified immunoglobulin or an antigen-binding fragment thereof, comprising the steps of:
(A) growing a cell line transformed with the nucleic acid of claim 1 under conditions in which the immunoglobulin or antigen-binding fragment thereof is expressed; and
(B) harvesting the expressed immunoglobulin or antigen-binding fragment thereof.