Devices for separation of biological materials
The present invention includes methods, devices and systems for isolating nanoparticulates, including nucleic acids, from biological samples. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity isolation of biological components from complex fluids such as blood or environmental samples.
1. A device for isolating a nanoscale analyte in a sample, the device comprising:
(a) a housing; and
(b) alternating current (AC) electrodes within the housing, wherein the AC electrodes are configured to be selectively energized to establish AC electrokinetic high field and AC electrokinetic low field regions, and the AC electrodes comprise conductive material configured within the AC electrodes for reducing, disrupting or altering fluid flow around or within the vicinity of the AC electrodes as compared to fluid flow in regions between or substantially beyond the vicinity, wherein the AC electrodes are configured in three-dimensions, and wherein the conductive material is present at the edge of the individual AC electrodes.
2. The device of claim 1 , wherein the conductive material is configured as discontinuous, curved lines in an open disk shape.
3. The device of claim 1 , wherein the conductive material is configured as a wavy line shape.
4. The device of claim 1 , wherein the conductive material is configured as a hollow triangular tube.
5. The device of claim 1 , wherein the conductive material is configured as a hollow ring with an extruded center.
6. The device of claim 1 , wherein the conductive material comprises a hydrogel coating.
7. A method for isolating a nanoscale analyte in a sample, the method comprising:
(a) applying the sample to a device, the device comprising an array of AC electrodes capable of establishing an AC electrokinetic high field and an AC electrokinetic low field, wherein the AC electrodes are configured in three-dimensions and comprise conductive material present at the edge of the individual AC electrodes which reduces, disrupts or alters fluid flow around or within the vicinity of the AC electrodes as compared to fluid flow in regions between or substantially beyond the vicinity;
(b) producing at least one AC electrokinetic field region, wherein the at least one AC electrokinetic field region is a dielectrophoretic high field region; and
(c) isolating the nanoscale analyte in the dielectrophoretic high field region.
8. The method of claim 7 , wherein the conductive material is only present at the edge of the individual electrodes in the array.
9. The method of claim 7 , wherein the conductive material is configured in three dimensions, increasing the total surface area of the conductive material within the electrodes.
10. The method of claim 7 , wherein the conductive material within the electrodes is configured at an angle.
11. The method of claim 7 , wherein the conductive material within the electrodes is configured into angles between neighboring planar electrode surfaces of less than 180 degrees or more than 60 degrees.
12. The method of claim 7 , wherein the conductive material within the electrodes is configured into a depressed concave shape.
13. The method of claim 7 , wherein the conductive material is configured as a hollow triangular tube.
14. The method of claim 7 , wherein the conductive material is configured as a hollow ring with an extruded center.
15. The method of claim 7 , wherein the electrodes are in a non-circular configuration.
16. The method of claim 15 , wherein an orientation angle between the non-circular configurations is between 25 and 90 degrees.
17. The method of claim 15 , wherein the non-circular configurations comprise a wavy line configuration, wherein a non-circular configuration comprises a repeating unit comprising a shape of a pair of dots connected by a linker, wherein the linker tapers inward toward the midpoint between the pair of dots, wherein the diameters of the dots are the widest points along the length of the repeating unit, wherein an edge to edge distance between a parallel set of repeating units is equidistant, or roughly equidistant.
18. The method of claim 7 , wherein the electrodes comprise one or more floating electrodes.
19. The method of claim 18 , wherein the floating electrodes are not energized to establish AC electrokinetic regions.
20. The method of claim 18 , wherein a floating electrode surrounds an energized electrode.
21. The method of claim 18 , wherein the floating electrodes in the array induce an electric field with a higher gradient than an electric field induced by non-floating electrodes in the array.
22. The method of claim 7 , wherein the conductive material comprises a hydrogel coating.