Tropoelastin for promoting endothelial cell adhesion or migration
The invention provides methods, compositions, and devices for promoting adhesion or migration of endothelial cell.
1. An implantable medical device coated with a fragment of human tropoelastin, wherein said fragment consists of 17 consecutive amino acids and has at least 80% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 6.
2. The device of claim 1 , wherein the human tropoelastin fragment is covalently attached to the device.
3. The device of claim 1 , wherein the human tropoelastin fragment is in an amount effective to promote adhesion of endothelial cells to the device.
4. The device of claim 3 , wherein the endothelial cells are endothelial stem cells or endothelial progenitor cells.
5. The device of claim 3 , wherein the amount of the human tropoelastin fragment is at least 100 ng per square centimeter.
6. The device of claim 1 , comprising a metal, an alloy or a plastic.
7. The device of claim 6 , wherein the metal comprises stainless steel.
8. The device of claim 1 , further comprising a biocompatible polymer.
9. The device of claim 8 , wherein the biocompatible polymer is selected from the group consisting of dacron, polyurethane, polypropylene, or polytetrafluoroethylene (PTFE).
10. The device of claim 1 , further comprising an anti-CD34 antibody.
11. The device of claim 10 , wherein the anti-CD34 antibody is monoclonal.
12. The device of claim 1 , wherein the device is a catheter, stent, shunt, wire, or other intraluminal device.
13. The device of claim 1 , wherein the device is a pacemaker, cardioverter-defibrillator, artificial valve, or vascular graft.
14. The device of claim 1 , comprising metal, plastic, silicone, dacron, polyurethane, polypropylene, PTFE, or derivatives thereof.