Synthetic peptide for repressing transcription and/or gene expression from a binding site of interest
View Patent ↗A synthetic peptide includes an amino acid sequence that: has 70-75%, 75-80%, 80-85%, 85-90%, 90-95% or 95-100% homology with an RpoN box of Region III of an RpoN protein; and binds specifically to a −24, −12, or −24/−12 site(s) of an RpoN promoter. The synthetic peptide is effective for repressing transcription and/or gene expression from a binding site of interest, and the binding site of interest is an RpoN binding site or a cryptic promoter upstream of an RpoN binding site.
1. A synthetic peptide comprising:
(a) an amino acid sequence wherein the amino acid sequence comprises SEQ ID NO: 10103 ; and
(b) said amino acid sequence binds specifically to a −24, −12, or −24/−12 site(s) of an RpoN promoter, wherein said synthetic peptide is effective for repressing transcription and/or gene expression from a binding site of interest, wherein the binding site of interest is an RpoN binding site or a cryptic promoter upstream of an RpoN binding site.
2. The synthetic peptide of claim 1 , wherein said amino acid sequence comprises SEQ ID NO:10103.
3. The synthetic peptide of claim 1 , further comprising a stabilized cyclic peptide or a stabilized fusion protein.
4. The synthetic peptide of claim 1 , further comprising maltose binding protein, glutathione, green fluorescent protein, small ubiquitin modifying protein, glutathione S-transferase, thioredoxin, ubiquitin, DsbA, NusA, bacterioferritin, or GrpE.
5. The synthetic peptide of claim 2 , further comprising a stabilized cyclic peptide or a stabilized fusion protein.
6. The synthetic peptide of claim 2 , further comprising maltose binding protein, glutathione, green fluorescent protein, small ubiquitin modifying protein, glutathione S-transferase, thioredoxin, ubiquitin, DsbA, NusA, bacterioferritin, or GrpE.
7. A peptide comprising the amino acid sequence of SEQ ID NO:
10103.
8. The synthetic peptide of claim 7 , further comprising a stabilized cyclic peptide or a stabilized fusion protein.
9. The synthetic peptide of claim 7 , further comprising maltose binding protein, glutathione, green fluorescent protein, small ubiquitin modifying protein, glutathione S-transferase, thioredoxin, ubiquitin, DsbA, NusA, bacterioferritin, or GrpE.
10. The peptide of claim 7 comprising the amino acid sequence of SEQ ID NO:10103.
11. The synthetic peptide of claim 10 , further comprising a stabilized cyclic peptide or a stabilized fusion protein.
12. The synthetic peptide of claim 10 , further comprising maltose binding protein, glutathione, green fluorescent protein, small ubiquitin modifying protein, glutathione S-transferase, thioredoxin, ubiquitin, DsbA, NusA, bacterioferritin, or GrpE.
13. A nucleic acid molecule comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:10103.
14. The nucleic acid molecule of claim 13 , further comprising a bacteriophage, a plasmid, a chromosomal insertion, a single-stranded RNA molecule, a single-stranded DNA molecule, a double-stranded DNA molecule, or a DNA-RNA hybrid molecule.
15. The nucleic acid molecule of claim 13 , wherein the nucleotide sequence comprises SEQ ID NO:10102.
16. The nucleic acid molecule of claim 15 , further comprising a bacteriophage, a plasmid, a chromosomal insertion, a single-stranded RNA molecule, a single-stranded DNA molecule, a double-stranded DNA molecule, or a DNA-RNA hybrid molecule.