Test cartridge with integrated transfer module
A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports.
1. A method comprising:
laterally translating a transfer module within a housing to align a structure on an outer surface of the transfer module with a first port associated with a first chamber and with a second port associated with a second chamber;
drawing a sample from the first chamber to the second chamber via at least the structure aligned over the first port and the second port; and
drawing the sample from the second chamber to a third chamber located within the transfer module via a port through a wall of the transfer module.
2. The method of claim 1 , further comprising mixing the sample introduced into the first chamber with a buffer disposed within the first chamber.
3. The method of claim 1 , further comprising mixing the sample drawn into the third chamber with a liquid already present in the third chamber.
4. The method of claim 1 , further comprising: introducing the sample to the first chamber via a swab carrying the sample.
5. The method of claim 1 , further comprising:
laterally translating the transfer module to align the port of the transfer module to a port of a fourth chamber;
drawing the sample into the fourth chamber from the third chamber; and
measuring one or more qualities of the sample while in the fourth chamber.
6. The method of claim 5 , wherein measuring comprises optically measuring a fluorescence signal.
7. The method of claim 5 , wherein measuring comprises optically measuring an absorbance.
8. The method of claim 5 , further comprising heating the sample after either drawing the sample into the third chamber or drawing the sample into the fourth chamber.
9. The method of claim 5 , wherein drawing the sample into the fourth chamber comprises flowing the sample through a filter.
10. The method of claim 5 , wherein drawing the sample into the fourth chamber comprises flowing the sample through a fluid splitter into one or more sub-chambers.
11. The method of claim 1 , further comprising repeating laterally translating the transfer module to align the port of the transfer module with various ports of one or more chambers.
12. The method of claim 9 , further comprising drawing one or more liquids into the third chamber from the one or more chambers via one or more pressure differentials.
13. The method of claim 1 , further comprising mixing the sample within the third chamber with a magnetic stir bar.