Methods and devices for detection and measurement of analytes
Sensors for target entities having functionalized thereon, at least one aptamer specific to the target entity, and methods of making and using the same are described for use in glycated protein monitoring and/or biomarkers.
1. A method for optimizing sensitivity and/or selectivity of a sensor for one or more analytes, comprising
linking one or more types of aptamers to a substrate with a self-assembled monolayer (SAM) linkage, the SAM linkage having a desired linking spacing and/or length to form a functionalized surface on the substrate,
wherein the SAM linkage is formed using dithiobis-N-succinimidyl propionate (DTSP) and (1-mercapto-11-undecyl) tri(ethylene glycol) (PEG3),
wherein PEG3 prevents non-specific adsorption of proteins, and
wherein a carboxylic moiety on DTSP forms a stable bonding with the aptamer.
2. The method of claim 1 , wherein at least one packing density and/or length of the SAM linkage affects a surface plasmon resonance (SPR) signal.
3. The method of claim 1 , wherein at least one packing density and/or length of the SAM linkage affects electrochemical impedance spectroscopy (EIS) signals.