Methods of inhibiting a bacterial virulence in a subject
Methods of inhibiting bacterial virulence in a subject are provided. The methods can include administering an effective amount of a formulation to inhibit a bacterial virulence in a subject, the formulation having a water soluble tannin combined with hydrogen peroxide in a pharmaceutically acceptable excipient; wherein, the tannin has a molecular weight ranging from about 170 Daltons to about 4000 Daltons; the tannin:peroxide weight ratio ranges from about 1:1000 to about 10:1; and, the composition inhibits a bacterial virulence in the subject when compared to a second subject in a control group in which the composition was not administered.
1. A method of inhibiting gastrointestinal spasm associated with a bacterial virulence in a subject, the method comprising:
administering an effective amount of a formulation to inhibit a bacterial virulence in the subject, the formulation having a water soluble tannin combined with hydrogen peroxide in a pharmaceutically acceptable excipient;
wherein,
the tannin has a molecular weight ranging from about 170 Daltons to about 4000 Daltons;
the tannin:peroxide weight ratio ranges from about 1:1000 to about 10:1; and,
the composition inhibits gastrointestinal spasm associated with a bacterial virulence in the subject when compared to a second subject in a control group in which the composition was not administered.
2. The method of claim 1 , wherein the tannin is a hydrolysable tannin.
3. The method of claim 1 , wherein the tannin is a water soluble condensed tannin.
4. The method of claim 1 , wherein the tannin is a mixture of a hydrolysable tannin and a water soluble condensed tannin.
5. The method of claim 1 , wherein the tannin is a flavanol.
6. The method of claim 1 , wherein the tannin is a catechin.
7. The method of claim 1 , wherein the tannin is gallic acid, epigallic acid, or a combination thereof.
8. The method of claim 1 , wherein the tannin is a gallotannin.
9. The method of claim 1 , wherein the tannin is an ellagitannin.
10. The method of claim 1 , wherein the tannin is a punicalagin.
11. The method of claim 1 , wherein the tannin is combined with the hydrogen peroxide as a component of a water extract of a plant tissue.
12. The method of claim 1 , wherein the tannin is combined with the hydrogen peroxide as a component of an alcohol extract of a plant tissue.
13. The method of claim 2 , wherein the hydrolysable tannin is a product of a water extraction of Chinese gall.
14. The method of claim 1 , wherein the tannin is a hydrolysable tannin having from 3-12 galloyl residues.
15. A method of inhibiting gastrointestinal spasm associated with a bacterial virulence in a subject, comprising:
administering an effective amount of a composition to a subject to inhibit a bacterial virulence; the formulation produced from a process including
combining a water soluble tannin with hydrogen peroxide at a tannin:peroxide weight ratio that ranges from about 1:1000 to about 10:1, the tannin having a molecular weight ranging from about 170 Daltons to about 4000 Daltons;
removing free hydrogen peroxide from the combination; and, mixing the combination of the tannin and the hydrogen peroxide with a pharmaceutically acceptable excipient to create the formulation;
wherein,
the administering includes selecting a desired concentration of the formulation for the administering; and,
the composition inhibits gastrointestinal spasm associated with a bacterial virulence in the subject when compared to a second subject in a control group in which the composition was not administered.
16. The method of claim 15 , wherein the tannin is a hydrolysable tannin.
17. The method of claim 15 , wherein the tannin is a water soluble condensed tannin.
18. The method of claim 15 , wherein the tannin is a mixture of a hydrolysable tannin and a water soluble condensed tannin.
19. The method of claim 15 , wherein the tannin is a flavanol.
20. The method of claim 15 , wherein the tannin is a catechin.
21. The method of claim 15 , wherein the tannin is gallic acid, epigallic acid, or a combination thereof.
22. The method of claim 15 , wherein the tannin is a gallotannin.
23. The method of claim 15 , wherein the tannin is an ellagitannin.
24. The method of claim 15 , wherein the tannin is a punicalagin.
25. The method of claim 15 , wherein the tannin is combined with the hydrogen peroxide as a component of a water extract of a plant tissue.
26. The method of claim 15 , wherein the tannin is combined with the hydrogen peroxide as a component of an alcohol extract of a plant tissue.
27. A method of inhibiting gastrointestinal spasm associated with a bacterial virulence in a subject, the method comprising:
administering an effective amount of a composition to a subject to inhibit a bacterial virulence in the subject, the composition having a water soluble polyphenol that was (i) water-extracted from pomegranate husk and green tea leaf and (ii) combined with hydrogen peroxide prior to the administering;
wherein, the hydrogen peroxide concentration ranges from about 0.001% to about 2.0% based on total dry weight of the composition;
the polyphenol comprises a tannin;
the polyphenol has a molecular weight ranging from about 170 Daltons to about 4000 Daltons; and,
the composition inhibits gastrointestinal spasm associated with a bacterial virulence in the subject when compared to a second subject in a control group in which the composition was not administered.
28. A method of inhibiting gastrointestinal spasm associated with a bacterial virulence in a subject, comprising:
administering an effective amount of a composition to inhibit a bacterial virulence in the subject,
the composition produced from a process including extracting a water soluble polyphenol from a combination of green tea leaf and pomegranate husk;
combining the polyphenol with hydrogen peroxide prior to the administering;
wherein, the polyphenol has a molecular weight ranging from about 170 Daltons to about 4000 Daltons;
selecting a desired concentration for the composition; and,
diluting the composition to the desired concentration for the administering;
wherein, the hydrogen peroxide concentration ranges from about 0.001% to about 2.0% based on total dry weight of the composition;
the polyphenol comprises a tannin; and,
the composition inhibits gastrointestinal spasm associated with a bacterial virulence in the subject when compared to a second subject in a control group in which the composition was not administered.