Methods and devices for preparation of enriched biological fluids
The present disclosure relates to methods and apparatus for producing platelet rich plasma, bone marrow mononuclear cells, stromal vascular fraction from adipose tissue, and other concentrated or enriched biological fluids.
1. A method of blood fractionation comprising:
a) centrifuging whole blood in a first syringe having a top coupling element and a bottom coupling element, wherein the whole blood fractionates into a platelet poor plasma layer, a buffy layer, and an erythrocyte layer within the syringe;
b) removing at least a portion of the platelet poor plasma layer from the top coupling element of the first syringe;
c) attaching a bottom coupling element of a second syringe or second container to the top coupling element of the first syringe;
d) drawing any of the platelet poor plasma layer, the buffy layer, and optionally part of the erythrocyte layer into the second syringe or second container; and
e) centrifuging the second syringe or second container wherein the any of the platelet poor plasma layer, the buffy layer, and the erythrocyte layer fractionates into a platelet rich plasma layer and an erythrocyte layer.
2. The method of claim 1 , wherein the platelet rich plasma layer is removed and transferred to a container.
3. The method of claim 1 , wherein the platelet rich plasma layer comprises about 8×10 6 to about 1×10 7 platelets/μL.
4. The method of claim 1 , wherein the platelet rich plasma layer comprises about 0.1×10 5 to about 2×10 5 erythrocytes/μL.
5. The method of claim 1 , wherein the platelet rich plasma layer comprises about 500 to about 4000 white blood cells/μL.
6. The method of claim 1 , wherein the platelet rich plasma layer comprises about 10 to about 300 neutrophils/μL.
7. The method of claim 1 , wherein the centrifuging in step (a) has a relative centrifugal force (RCF) of about 1500×g to about 2000×g.
8. The method of claim 1 , wherein the centrifuging in step (e) has a relative centrifugal force (RCF) of about 30×g to about 200×g.
9. The method of claim 1 , wherein the second syringe is a double syringe.
10. The method of claim 9 , wherein the double syringe comprises an outer syringe having a first body with a first diameter; and an inner syringe having a second body with a second diameter smaller than the first diameter, the outer syringe being in direct fluid communication with the inner syringe, and the inner syringe being located within the distal outer syringe.
11. The method of claim 10 , wherein the inner syringe comprises a plunger, and wherein the plunger of the inner syringe is pulled up to isolate the platelet rich plasma after the second centrifugation without disturbing the erythrocyte layer.
12. The method of claim 11 , wherein the inner syringe is removed from the outer syringe and a needle is attached to a bottom coupling element of the inner syringe.
13. The method of claim 12 , further comprising injecting the platelet rich plasma from the inner syringe directly into a patient or container.
14. The method of claim 1 , further comprising expelling the erythrocyte layer from the second syringe or second container and retaining the platelet rich plasma in the second syringe or second container.
15. The method of claim 14 , further comprising attaching a needle to the bottom coupling element of the second syringe or second container.
16. The method of claim 15 , further comprising injecting the platelet rich plasma of the second syringe into a ligament, tendon, bone, cartilage, or joint space of a patient.