Method of distributing discrete polymer networks

1. A method of preparing a discrete polymer network array, the method comprising:

mixing a plurality of nucleic acid discrete polymer networks with a plurality of color-activated polymer networks to form a dispersion, the color-activated polymer networks free of nucleic acids, wherein the color-activated polymer network includes a dye having negatively charged terminal groups;

applying the dispersion to an array of wells disposed over chemical field-effect transistors, the nucleic acid discrete polymer networks selectively depositing into wells of the array of wells; and

rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

2. The method of claim 1 , wherein the color-activated polymer networks are smaller than openings of the wells of the array of wells.

3. The method of claim 1 , wherein the color-activated polymer networks include a hydrophilic polymer.

4. The method of claim 1 , wherein the hydrophilic polymer includes a hydrogel polymer.

5. The method of claim 1 , wherein the color-activated polymer networks is selected from a group consisting of a polyacrylamide, a polyethylene glycol, and a copolymer.

6. The method of claim 1 , wherein the nucleic acid discrete polymer networks have an average discrete polymer network size in a range of 0.1 microns to 10 microns.

7. The method of claim 6 , wherein the average discrete polymer network size is in a range of 0.1 microns to 0.8 microns.

8. The method of claim 1 , wherein the color-activated polymer networks have an average discrete polymer network size in a range of 0.1 microns to 10 microns.

9. The method of claim 8 , wherein the average discrete polymer network size is in range of 0.1 microns to 2 microns.

10. The method of claim 1 , further comprising washing the dispersion prior to applying the dispersion to the array of wells.

11. The method of claim 1 , wherein the array of wells comprises a sequencing device, the method further comprising sequencing a polynucleotide coupled to the nucleic acid discrete polymer network.

12. The method of claim 1 , further comprising amplifying a polynucleotide target on the nucleic acid discrete polymer network.

13. The method of claim 1 , wherein fewer than 5% of the wells include a color-activated polymer network following application of the dispersion.

14. The method of claim 13 , wherein fewer than 2% of the wells include a color-activated polymer network following application of the dispersion.

15. The method of claim 14 , wherein fewer than 1% of the wells include a color-activated polymer network following application of the dispersion.

16. The method of claim 1 , wherein the nucleic acid discrete polymer networks deposit with a selectivity ratio of at least 10 relative to the color-activated polymer networks.

17. The method of claim 16 , the selectivity ratio is at least 20.

18. The method of claim 1 , further comprising:

pelleting the dispersion to form a pellet including the plurality of nucleic acid discrete polymer networks and the plurality of color-activated polymer networks;

washing the pellet; and

redispersing the pellet to reform the dispersion.