IP Library › Granted Patent US 10,316,068
Granted Patent B2
US 10,316,068 · App. 15/210,834 · Granted Jun 11, 2019

Synthetic polypeptide having a xylose import activity

Inventors: Aindrila Mukhopadhyay (Oakland, CA); Amanda Reider Apel (Alameda, CA); Mario Ouellet (Castro Valley, CA); Jay D. Keasling (Berkeley, CA)
Assignee: The Regents of the University of California
C07K14/395
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Quick Facts
Patent No.
US 10,316,068
App. No.
15/210,834
Granted
Jun 11, 2019
Kind
B2
Abstract

This disclosure provides methods and compositions related to microbial gene expression. In one aspect, a synthetic polypeptide having a xylose import activity.

Claims (5)

1. A synthetic polypeptide comprising an amino acid sequence identical to SEQ ID NO: 2 except there is an amino acid with a polar side chain at position 79 and wherein said synthetic polypeptide has a xylose import activity.

2. The synthetic polypeptide of claim 1 , wherein the amino acid with a polar side chain is selected from the group consisting of: glycine, serine, threonine, cysteine, tyrosine, asparagine, glutamine, lysine, arginine, histidine, aspartic acid, and glutamine acid.

3. The synthetic polypeptide of claim 2 , wherein the amino acid with a polar side chain is serine.

4. The synthetic polypeptide of claim 1 , wherein the xylose import activity has a Vmax having a value within from 166.30 to 206.52 nmol·min −1 ·mg −1 and a Km having a value within from 182.9 to 274.7 mM.

5. The synthetic polypeptide of claim 4 , wherein the xylose import activity has a Vmax having a value of at least 186.41 nmol·min −1 ·mg −1 and a Km having a value of at least 228.8 mM.

Assignments (2)
CONFIRMATORY LICENSE Recorded Aug 3, 2016
From: REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE
To: ENERGY, UNITED STATES DEPARTMENT OF
Reel/Frame 039627/0480 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 27, 2016
From: MUKHOPADHYAY, AINDRILA; REIDER APEL, AMANDA R.; OUELLET, MARIO; KEASLING, JAY D.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 039273/0303 →
Continuity (2)
Provisional Application 62192517 · Jul 14, 2015
Related Publication 20170015714A1 · Jan 19, 2017