Biohybrid composite scaffold
A biohybrid scaffold is provided that is useful in clinical applications for abdominal wall reconstruction, pelvic floor repair, breast reconstruction, as well as other soft tissue repairs. Methods of making and using the biohybrid scaffold are provided.
1. A biohybrid scaffold comprising a matrix of a biodegradable, biocompatible elastomeric polymer and an ECM-derived gel interspersed substantially evenly throughout the matrix, wherein
the ECM-derived gel composition is prepared by: (i) solubilizing decellularized tissue that has not been dialyzed by digestion with an acid protease, thereby producing a digest solution; and (ii) raising the pH of the digest solution to between 7.2 and 7.8.
2. The biohybrid scaffold of claim 1 , wherein the ECM-derived gel has a lower critical solution temperature (LCST) of less than 37° C.
3. The biohybrid scaffold of claim 2 , wherein the ECM-derived gel has an LCST of from 20° C. to less than 37° C.
4. The biohybrid scaffold of claim 1 , wherein the biodegradable, biocompatible elastomeric polymer comprises a poly(ester urethane) urea (PEUU), a poly(ether ester urethane)urea (PEEUU), a poly(ester carbonate)urethane urea (PECUU), and/or a poly(carbonate)urethane urea (PCUU).
5. The biohybrid scaffold of claim 1 , in which the biodegradable, biocompatible elastomeric polymer comprises a copolymer of polycaprolactone, 1,4-diisocyanobutane, and putrescine.
6. The biohybrid scaffold of claim 1 , further comprising one or more layers of a wet-electrospun biodegradable, biocompatible elastomeric polymer attached to the matrix of a biodegradable, biocompatible elastomeric polymer and the ECM-derived gel interspersed substantially evenly throughout the matrix, forming a composite scaffold structure.
7. The biohybrid scaffold of claim 6 , wherein the composite scaffold comprises the matrix of a biodegradable, biocompatible elastomeric polymer and an ECM-derived gel interspersed substantially evenly throughout the matrix sandwiched between two layers of the wet-electrospun biodegradable, biocompatible elastomeric polymer.
8. The biohybrid scaffold of claim 6 , wherein the wet-electrospun biodegradable, biocompatible elastomeric polymer comprises a PEUU and PBS.
9. The biohybrid scaffold of claim 6 , in which polymer fibers between the one or more layers of a wet-electrospun biodegradable, biocompatible elastomeric polymer and the matrix of a biodegradable, biocompatible elastomeric polymer and the ECM-derived gel interspersed substantially evenly throughout the matrix are interlocked or continuous between the layers.
10. A method of growing tissue in a patient comprising implanting a biohybrid scaffold comprising a matrix of a biodegradable, biocompatible elastomeric polymer and an ECM-derived gel interspersed substantially evenly throughout the matrix in a patient at a site of injury or defect in the patient, wherein
the ECM-derived gel composition is prepared by: (i) solubilizing decellularized tissue that has not been dialyzed by digestion with an acid protease, thereby producing a digest solution; and (ii) raising the pH of the digest solution to between 7.2 and 7.8.
11. The method of claim 10 , wherein the biohybrid scaffold is implanted to repair a soft-tissue injury or defect in a patient.
12. The method of claim 11 , wherein the biohybrid scaffold is implanted in the abdominal wall, the pelvic floor, or a breast of a patient, thereby repairing an injury or defect in the patient.
13. The method of claim 11 , wherein the biohybrid scaffold is implanted in an abdominal wall the patient, thereby repairing an injury or defect in the abdominal wall of the patient.
14. A biohybrid scaffold comprising:
a matrix of a biodegradable, biocompatible elastomeric polymer;
an ECM-derived gel interspersed substantially evenly throughout the matrix; and
one or more layers of a wet-electrospun biodegradable, biocompatible elastomeric polymer attached to the matrix of a biodegradable, biocompatible elastomeric polymer and the ECM-derived gel interspersed substantially evenly throughout the matrix, forming a composite scaffold structure.
15. The biohybrid scaffold of claim 14 , wherein the composite scaffold comprises the matrix of a biodegradable, biocompatible elastomeric polymer and an ECM-derived gel interspersed substantially evenly throughout the matrix sandwiched between two layers of the wet-electrospun biodegradable, biocompatible elastomeric polymer.
16. The biohybrid scaffold of claim 14 , wherein the wet-electrospun biodegradable, biocompatible elastomeric polymer comprises a PEUU and PBS.
17. The biohybrid scaffold of claim 14 , in which polymer fibers between the one or more layers of a wet-electrospun biodegradable, biocompatible elastomeric polymer and the matrix of a biodegradable, biocompatible elastomeric polymer and the ECM-derived gel interspersed substantially evenly throughout the matrix are interlocked or continuous between the layers.
18. A method of growing tissue in a patient comprising:
implanting in a patient at a site of injury or defect in the patient a biohybrid scaffold comprising:
a matrix of a biodegradable, biocompatible elastomeric polymer;
an ECM-derived gel interspersed substantially evenly throughout the matrix; and
one or more layers of a wet-electrospun biodegradable, biocompatible elastomeric polymer attached to the matrix of a biodegradable, biocompatible elastomeric polymer and the ECM-derived gel interspersed substantially evenly throughout the matrix, forming a composite scaffold structure.
19. The method of claim 18 , wherein the biohybrid scaffold is implanted to repair a soft-tissue injury or defect in a patient.
20. The method of claim 19 , wherein the biohybrid scaffold is implanted in the abdominal wall, the pelvic floor, or a breast of a patient, thereby repairing an injury or defect in the patient.