Immunotherapy against several tumors including neuronal and brain tumors
The present invention relates to peptides, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated cytotoxic T cell (CTL) peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention relates to 30 peptide sequences and their variants derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.
1. A peptide consisting of the amino acid sequence of NLDTLMTYV (SEQ ID NO: 1) in the form of pharmaceutically acceptable salt, wherein said peptide is produced by solid phase peptide synthesis or produced by a yeast cell or bacterial cell expression system.
2. A kit comprising:
(a) a container that contains a composition containing, in solution and/or in lyophilized form, the peptide salt of claim 1 ;
(b) optionally, a second container comprising a diluent and/or reconstituting solution;
(c) optionally, at least one additional peptide consisting of the amino acid sequence selected from the group consisting of SEQ ID NOs 2-30, and
(d) optionally, instructions for (i) use of the diluent and/or (ii) reconstitution and/or use of a lyophilized formulation.
3. The kit according to claim 2 , further comprising one or more of (e) a buffer, (f) a diluent, (g) a filter, (h) a needle, and (i) a syringe.
4. An acylated peptide consisting of the amino acid sequence of NLDTLMTYV (SEQ ID NO: 1) or a pharmaceutically acceptable salt thereof.
5. A pegylated peptide consisting of the amino acid sequence of NLDTLMTYV (SEQ ID NO: 1) or a pharmaceutically acceptable salt thereof.
6. A composition comprising the peptide of claim 1 and a pharmaceutically acceptable carrier.
7. A composition comprising the peptide of claim 1 produced by solid phase peptide synthesis, wherein the peptide is linked to a solid support.
8. A composition comprising the acylated peptide of claim 4 or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
9. A composition comprising the pegylated peptide of claim 5 or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
10. The peptide of claim 1 , wherein the pharmaceutically acceptable salt is a chloride salt or acetate salt.
11. The peptide of claim 1 , wherein said peptide is produced by solid phase peptide synthesis using a solid-phase support followed by removal from the solid-phase support.
12. The peptide of claim 11 , wherein said peptide is produced by solid phase peptide synthesis using a solid-phase support followed by removal from the solid-phase support by a composition comprising 95% trifluoroacetic acid and a 50% scavenger mix.
13. The peptide of claim 12 , further comprising removing the excess trifluoroacetic acid by evaporation.
14. The peptide of claim 13 , further comprising purifying the peptide using a method selected from the group consisting of re-crystallization, size exclusion chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high performance liquid chromatography.
15. The peptide of claim 14 , wherein the purification is performed using ion-exchange chromatography using an organic or inorganic acid.
16. The peptide of claim 1 , which contains at least one D-amino acid.