Recombinant turkey herpesvirus vaccines and uses thereof
The present disclosure provides a recombinant viral vector comprising at least one transgene inserted into a Marek's disease viral genome for treatment of diseases in poultry. Also provided are immunogenic compositions comprising such recombinant viral vectors and methods for preventing or inhibiting Marek's disease in combination with at least a second disease in poultry.
1. A recombinant viral vector comprising at least one transgene inserted into a Marek's disease viral genome in an intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome.
2. A recombinant viral vector comprising at least one transgene inserted into a Marek's disease viral genome in a region selected from the group consisting of:
(a) an intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome; and
(b) an intergenic region flanked by HVT86 (US10) and HVT87 (Sorf3) in the unique short region of the genome.
3. The recombinant viral vector of claim 2 , wherein the at least one transgene expresses an antigenic viral gene.
4. The recombinant viral vector of claim 2 , wherein the at least one transgene comprises a first transgene inserted into the intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome, and a second transgene inserted into the intergenic region flanked by HVT86 (US10) and HVT87 (Sorf3) in the unique short region of the genome.
5. The recombinant viral vector of claim 2 , wherein the at least one transgene comprises more than one transgene inserted in both the intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome, and the intergenic region flanked by HVT86 (US10) and HVT87 (Sorf3) in the unique short region of the genome.
6. The recombinant viral vector of claim 3 , wherein the antigenic viral gene comprises a gene selected from the group consisting of an infectious bursal disease virus gene, a Newcastle disease virus gene, an avian influenza virus gene, and an infectious laryngotracheitis virus gene.
7. The recombinant viral vector of claim 6 , wherein the infectious bursal disease virus gene is a VP2 gene, or the Newcastle disease virus gene is an F gene or an HN gene or an F/HN chimera.
8. The recombinant viral vector of claim 2 , wherein the at least one transgene is operatively linked to a heterologous promoter.
9. The recombinant viral vector of claim 8 , wherein the promoter comprises a promoter selected from the group consisting of a human cytomegalovirus IE promoter, a guinea pig CMV promoter, an SV40 promoter, a Pseudorabies Virus promoter, a glycoprotein X promoter, a Herpes Simplex Virus-1 promoter, and a Marek's disease viruses promoters.
10. The recombinant viral vector of claim 9 , wherein the promoter comprises a human cytomegalovirus IE promoter.
11. The recombinant viral vector of claim 2 , wherein the at least one transgene is operatively linked to a polyA signal.
12. The recombinant viral vector of claim 11 , wherein the polyA signal is selected from the group consisting of a bovine growth hormone polyA signal, an SV40 polyA signal, an AcNPV 1629 ORF poly(A) signal, and an HSV TK polyA signal.
13. The recombinant viral vector of claim 12 , wherein the polyA signal is a bovine growth hormone polyA signal.
14. The recombinant viral vector of claim 2 , wherein the at least one transgene is inserted into a Marek's disease viral genome in an intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome.
15. An immunogenic composition comprising the recombinant viral vector of claim 2 .
16. The immunogenic composition of claim 15 , wherein the at least one transgene comprises a first transgene inserted into the viral genome in an intergenic region flanked by HVT10 (UL3) and HVT11 (UL4) in the unique long region of the genome; and a second transgene inserted into the viral genome in an intergenic region flanked by HVT86 (US10) and HVT87 (Sorf3) in the unique short region of the genome.
17. The immunogenic composition of claim 16 , further comprising at least a third transgene conferring protection against a third disease.
18. The immunogenic composition of claim 15 , wherein the at least one transgene is operatively linked to a heterologous promoter.
19. The immunogenic composition of claim 16 , wherein the first and second transgenes are operatively linked to the same promoter.
20. The immunogenic composition of claim 16 , wherein the first transgene is operatively linked to a heterologous promoter and the second transgene is operatively linked to a second heterologous promoter.
21. The immunogenic composition of claim 15 , wherein the at least one transgene encodes a viral gene selected from the group consisting of an infectious bursal disease virus gene, a Newcastle disease virus gene, an avian influenza virus gene, and an infectious laryngotracheitis virus gene.
22. The immunogenic composition of claim 21 , wherein the infectious bursal disease virus gene is a VP2 gene, or the Newcastle disease virus gene is an F gene or an HN gene or an F/HN chimera.
23. A method for preventing or inhibiting Marek's disease in combination with at least a second disease in poultry, comprising providing the composition of claim 15 to a bird, wherein the composition is provided in an amount effective to prevent or inhibit Marek's disease and the at least a second disease in the bird.
24. The method of claim 23 , wherein the composition is provided to the bird by injection.
25. The method of claim 24 , wherein the injection is selected from the group consisting of intravenous injection, intramuscular injection, subcutaneous injection, and in ovo injection.
26. The method of claim 23 , wherein the composition is provided to the bird prior to infection with or exposure to a disease.
27. The method of claim 23 , wherein the bird is a species of poultry.
28. The method of claim 27 , where the species of poultry is selected from the group consisting of a chicken, a turkey, a quail, a goose, a duck, a swan, a guinea, and a pigeon.
29. The method of claim 23 , wherein the composition is provided to the bird in combination with a non-naturally occurring pharmaceutically acceptable carrier.