Adapted rhinovirus C
A mutated rhinovirus C, methods of creating and methods of propagating thereof, wherein the mutated rhinovirus shows enhanced virus yields after infection and induced visible cytopathic effect.
1. An isolated mutated rhinovirus C, wherein the mutation is E 41 K in rhinovirus C protein 3A or a mutation in the amino acid at a positional equivalent of E 41 in protein 3A to K in non-C15 strains.
2. The virus of claim 1 , wherein the rhinovirus C is a clinical isolate selected from the group consisting of C15, C41, and C2.
3. The virus of claim 2 , wherein the rhinovirus C is clinical isolate C15 and the mutation comprises E 41 K in rhinovirus C protein 3A.
4. The virus of claim 2 , wherein the rhinovirus C is clinical isolate C15 and the mutation comprises both E 41 K in rhinovirus C protein 3A and T 125 K in rhinovirus C protein VP1.
5. The virus of claim 2 , wherein the rhinovirus C is clinical isolate C41 and the mutation is E 41 K in rhinovirus C protein 3A in strain C41.
6. The virus of claim 2 , wherein the rhinovirus C is clinical isolate C2 and the mutation is E 41 K in rhinovirus C protein 3A in strain C2.
7. The virus of claim 1 , wherein the mutated rhinovirus C additionally comprises a reporter.
8. The virus of claim 7 , wherein the reporter is selected from the group consisting of DsRed and GFP.
9. The virus of claim 7 , wherein the reporter is positioned upstream of a viral open reading frame and linked by SEQ ID NO:1.
10. The virus of claim 7 , wherein the reporter is between VP1 and 2A and linked by SEQ ID NO:2.
11. A method of propagating rhinovirus C, comprising the step of infecting a transduced cell line expressing CDHR3 with the mutated rhinovirus C of claim 1 .
12. The method of claim 11 , wherein the cell line is a HeLa-E8 cell line.
13. The method of claim 11 , wherein the rhinovirus C is a clinical isolate selected from the group consisting of C15, C41, and C2.
14. The method of claim 13 , wherein the rhinovirus C is clinical isolate C15 and the mutation comprises both T 125 K in rhinovirus C protein VP1 and E 41 K in rhinovirus C protein 3A.
15. A method of creating a mutated rhinovirus C, wherein the method comprises the steps of introducing E 41 K in rhinovirus C protein 3A of strain C15 or a mutation that is a positional equivalent of E 41 K in non-C15 strains, and isolating the mutated rhinovirus C.
16. An isolated mutated rhinovirus C produced by the method of claim 15 .