Method of generating human pancreatic cells
The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy.
1. A method for generating human pancreatic cells comprising the steps of:
i) culturing human embryoid bodies in the presence of a concentration of 100 ng/ml of activin such that a cell population enriched for endoderm is obtained; and ii) differentiating the cell population enriched for endoderm obtained in step i) under conditions such that human pancreatic cells are generated.