Methods of differentiating stem cells by modulating miR-124
Provided herein are methods of differentiating stem cells via modulating miR-124, and the differentiated cells thereby. Also provided herein are methods for the treatment of diseases using the differentiated cells.
1. An isolated pancreatic progenitor cell, wherein the isolated pancreatic progenitor cell expresses insulin and betatrophin.
2. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell further expresses C-peptide.
3. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell further expresses Ngn3.
4. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell is differentiated from a human trophoblast stem cell.
5. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has a normal karyotype compared to the trophoblast stem cell.
6. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has low or absence of CD133 expression compared to the trophoblast stem cell.
7. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell is differentiated from a trophoblast stem cell, and wherein the isolated pancreatic progenitor cell has low or absence of CD133 expression compared to the trophoblast stem cell.
8. The isolated pancreatic progenitor cell of claim 1 , wherein the isolated pancreatic progenitor cell further expresses C-peptide and Ngn3.