Graphene oxide mediated cellular delivery of gadolinium-labeled molecules
A method of bioactive molecule delivery includes providing a first aqueous medium comprising native graphene oxide and a second aqueous medium comprising a bioactive molecular component that includes Gd(III)-labeled molecules; mixing said first and second media to form a mixture thereof; co-incubating the mixture for a first period of time for coupling said molecular component on a surface of said native graphene oxide, to provide a co-incubation product; and contacting a cellular medium with said co-incubation product for a second period of time for cellular delivery of said bioactive molecular component.
1. A method of bioactive molecule delivery, comprising:
incubating cells with a co-incubation medium for 24 hours to deliver a bioactive molecular component into the cells, wherein said co-incubation medium comprises functionalized graphene oxide (GO) having said bioactive molecular component adsorbed on a surface of graphene oxide, wherein said bioactive molecular component consists of Gd(III)-labeled molecules; and
enhancing delivery of said bioactive molecular component into the cells by GO sedimentation.
2. The method of claim 1 , wherein said Gd(III)-labeled molecules comprise a moiety selected from alkyl, amine, hydroxy, phosphate, carboxy, quaternary amine and aminoalkyl moieties, and combinations thereof.
3. The method of claim 1 , wherein said graphene oxide has a lateral surface dimension of less than 1500 nm.
4. The method of claim 3 , wherein said lateral surface dimension is 150 nm.
5. A method of bioactive molecule delivery, comprising:
incubating cells with a co-incubation medium for a period of time to deliver a bioactive molecular component into the cells, wherein said co-incubation medium comprises functionalized graphene oxide (GO) having said bioactive molecular component adsorbed on a surface of graphene oxide, wherein said bioactive molecular component consists of Gd(III)-labeled molecules, and wherein the concentrations of Gd(III) and GO in said co-incubation medium are 188 μM and 18.8 μg/mL, respectively.
6. The method of claim 1 , wherein said cells comprise HeLa cells and/or KB cells.
7. The method of claim 1 , wherein said co-incubation medium has a Gd(III)-labeled molecule-to-GO ratio of 2.1×10 6 Gd/10 5 C.
8. The method of claim 5 , wherein the period of time is 24 hours.