Bone matrix compositions and methods for their preparation and use
The present disclosure provides novel bone matrix (BM) materials, and methods for preparation and use of the demineralized bone matrix materials and compositions therefrom. The methods of preparation include the use of a closed-vessel demineralization system, as well as simple, easily reproducible process steps, and significantly shortened times for demineralization. These novel BM materials do not require the inclusion of carriers and/or delivery agents, or the addition of binders, and can be made in various forms, including a clay, putty, crush, powder, or gel. The novel BM compositions and methods disclosed herein may, illustratively, have applications in the medical field, such as in surgical bone graft applications, in the repair and/or regeneration of bone and bone-related tissue, and the like.
1. A bone matrix composition consisting of:
bone powder;
brushite particles; and
monetite particles;
wherein the bone powder, brushite particles, and monetite particles are combined to yield an admixture;
wherein the bone powder, brushite, and monetite are derived from bone;
wherein application of shear force to the admixture yields a self-binding putty;
wherein the self-binding putty exhibits sufficient wet-field integrity to be non-sticky and to resist degradation during lavage; and
wherein the self-binding putty exhibits decreasing viscosity with increased application of shear force; and
wherein the admixture is 16-50% by weight monetite and 16-80% by weight brushite.
2. The bone matrix composition of claim 1 , wherein the ratio of brushite to monetite present is from about 2.2 to about 0.25.
3. The bone matrix composition of claim 1 , wherein the bone matrix composition contains less than 8 volume percent calcium.
4. The bone matrix composition of claim 1 , wherein the bone powder is demineralized.
5. The bone matrix composition of claim 1 , wherein the admixture particle size ranges from 200 microns to 800 microns.
6. A bone matrix composition consisting of:
bone powder;
brushite particles; and
monetite particles;
wherein the composition includes a physiological compilation of bioavailable growth factors;
wherein the bone powder, brushite particles, and monetite particles are combined to yield an admixture;
wherein the bone powder, brushite, and monetite are derived from bone;
wherein application of shear force to the admixture yields a self-binding putty;
wherein the self-binding putty exhibits sufficient wet-field integrity to be non-sticky and to resist degradation during lavage; and
wherein the self-binding putty exhibits decreasing viscosity with increased application of shear force; and
wherein the admixture is 16-50% by weight monetite and 16-80% by weight brushite.
7. The bone matrix composition of claim 6 , wherein the physiologic compilation of bioavailable growth factors includes bone morphogenic proteins.
8. The bone matrix composition of claim 7 , wherein the bone morphogenic proteins are selected from the group comprising BMP-2, BMP-4, BMP-7, VEGF, and combinations thereof.
9. A bone matrix composition, consisting of:
an organic phase; and
a particulate mineral phase, consisting of:
16-50% by weight monetite;
16-80% by weight brushite;
hydroxyapatite;
wherein the particulate mineral phase comprises 46-74% by weight of the bone matrix.
10. The bone matrix composition of claim 9 , wherein the application of shear force to the composition converts the composition from a powder to a putty.
11. The bone matrix composition of claim 10 , wherein the composition is a putty after sufficient shear stress is applied.
12. The bone matrix composition of claim 9 , wherein the particle size range of the particulate mineral phase is from 200 microns to 800 microns.
13. A human bone matrix composition consisting of:
demineralized bone matrix containing both monetite and brushite;
wherein said composition maintains osteoinductive, osteoconductive, and osteogenic properties;
wherein said composition is a putty that exhibits wet field integrity including self-binding;
and wherein the composition is 16-50% by weight monetite and 16-80% by weight brushite.