Bacteria and enzymes produced therefrom and methods of using same
A bacteria referred to here as Bacillus subtilis 6A-1 is provided, compositions thereof and processes for use of the bacteria, spores, cells, extracts and enzymes. The compositions which comprise the bacteria, spores, cells, extracts and/or enzymes are capable of degrading polysaccharides. Such compositions are capable of degrading cellulose, including plant-produced cellulose, microcrystalline cellulose and carboxymethyl cellulose. The bacteria produces at least two cellulose-degrading protein fractions. Cellulose degrading activity continues across pH2 to pH13.
1. A method of producing a composition capable of degrading at least one polysaccharide, the method comprising,
a) culturing strain Bacillus subtilis 6A-1 (6A-1), reference culture comprising said 6A-1 having been deposited at ATCC under deposit number PTA-125135, such that said 6A-1 produces at least two polysaccharide-degrading protein fractions;
b) a process selected from (i) filtering or drying or freeze drying or grinding, or adding at least one excipient, carrier or diluent to said 6A-1 strain or cells or spores of said 6A-1 or (ii) extracting at least one polysaccharide-degrading protein fraction from said 6A-1, or iii) a combination thereof and
c) producing a composition comprising said strain, cells, spores or at least one polysaccharide-degrading protein fraction extraction of b), or a combination thereof.
2. The method of claim 1 , wherein said composition comprises at least three cellulose-degrading protein fractions produced by said 6A-1, said protein fractions capable of degrading crystalline cellulose, carboxymethyl cellulose and unmodified cellulose.
3. The method of claim 1 , wherein said composition is capable of degrading protein at a pH of 2 to 12.
4. The method of claim 1 , wherein said 6A-1 is an asporogenous mutant of said 6A-1.
5. The method of claim 1 , wherein said composition is capable of degrading cellulose under conditions from pH 2 to pH 13.
6. The method of claim 1 , wherein said composition is capable of degrading acid detergent fiber.
7. The method of claim 1 , wherein said 6A-1 is cultured at a temperature of less than 55° C.
8. The method of claim 1 , wherein said 6A-1 is cultured at a temperature of 30°-35° C.
9. The method of claim 1 , wherein said 6A-1 is cultured in a liquid or semi-solid medium.
10. A method of producing an edible composition for an animal, the method comprising,
a) culturing strain Bacillus subtilis 6A-1 (6A-1), reference culture comprising said 6A-1 having been deposited at ATCC under deposit number PTA-125135 such that said 6A-1 produces at least two polysaccharide-degrading protein fractions;
b) a process selected from (i) filtering or drying or freeze drying or grinding, or adding at least one excipient, carrier or diluent to said 6A-1 strain or cells or spores of said 6A-1 or (ii) extracting at least one polysaccharide-degrading protein fraction from said 6A-1, or iii) a combination thereof; and
c) producing an edible composition selected from
(i) a composition comprising said strain, cells, spores or at least one polysaccharide-degrading protein fraction or combination thereof of b); or
(ii) a composition comprising one or more plants or plant parts fermented with said strain, cells spores or at least one polysaccharide-degrading protein fraction or combination thereof of b) until said fermentation is complete.
11. The method of claim 10 , wherein b) comprises drying said 6A-1 strain, cells, spores or at least one polysaccharide-degrading protein fraction or combination thereof and further comprises spraying said dried strain cells, spores or at least one polysaccharide-degrading protein fraction or combination thereof onto one or more plants or plant parts.
12. The method of claim 10 , comprising producing a probiotic edible composition comprising said strain, cells, spores or at least one polysaccharide-degrading protein fraction or combination thereof of b).
13. A method of feeding an animal, the method comprising,
a) culturing strain Bacillus subtilis 6A-1 (6A-1), reference culture comprising said 6A-1 having been deposited at ATCC under deposit number PTA-125135 such that said 6A-1 produces at least two polysaccharide-degrading protein fractions;
b) a process selected from (i) filtering or drying or freeze drying or grinding, or adding at least one excipient, carrier or diluent to said 6A-1 strain or cells or spores of said 6A-1 or (ii) extracting at least one polysaccharide-degrading protein fraction from said 6A-1, or iii) a combination thereof;
c) feeding said animal an edible composition selected from,
(i) said strain, cells, spores or at least one polysaccharide-degrading protein fraction or combination thereof of b); or
(ii) a composition comprising one or more plants or plant parts fermented with said strain, cells spores or at least one polysaccharide-degrading protein fraction or combination thereof of b) until said fermentation is complete.
14. The method of claim 13 , wherein said polysaccharide degrading protein fractions retain said polysaccharide degrading activity at a pH of 2 to 13.
15. The method of claim 13 , wherein said animal is selected from a lactating animal, ruminant, lactating ruminant, poultry, swine, equine, canine, feline, or rabbit.
16. The method of claim 13 , wherein said edible composition comprises said plants or plant parts.
17. The method of claim 16 , wherein dry matter digestibility, neutral detergent digestibility, acid detergent fiber digestibility or hemicellulose digestibility or a combination thereof of said edible composition is increased after said fermentation is complete.
18. The method of claim 16 , wherein lactic acid is lower, pH is lower, or ammonia concentration lower, or a combination thereof of said edible composition after said fermentation is complete.
19. The method of claim 1 , wherein said strain, cells, spores or at least one polysaccharide-degrading protein fraction is dried or freeze dried.
20. The method of claim 1 , wherein said composition comprises said strain.
21. The method of claim 1 , wherein said composition comprises said cells or spores.
22. The method of claim 1 , wherein said at least one excipient, carrier or diluent is edible.
23. The method of claim 1 , wherein said composition is edible.