Surgical sutures incorporated with stem cells or other bioactive materials
Materials and methods for immobilizing bioactive molecules, stem and other precursor cells, and other agents of therapeutic value in surgical sutures and other tissue scaffold devices are described herein. Broadly drawn to the integration and incorporation of bioactive materials into suture constructs, tissue scaffolds and medical devices, the present invention has particular utility in the development of novel systems that enable medical personnel performing surgical and other medical procedures to utilize and subsequently reintroduce bioactive materials extracted from a patient (or their allogenic equivalents) to a wound or target surgical site.
1. A method of making a surgical suture or an elongate linear tissue scaffold having an exterior surface and interior core comprised of a matrix of interwoven filaments or a porous monofilament, wherein the exterior surface comprises constrictions at intervals along the longitudinal axis and wherein a concentration of a bioactive material selected from the group consisting of biological cells and therapeutic agents is dispersed throughout said interior core, said method comprising the steps of:
compressing or otherwise manipulating said matrix along or around a longitudinal axis so as to deform the interstices between interwoven filaments or the pores of the monofilament to a first size and shape sufficient to permit the migration of said bioactive material from a surrounding media, across said exterior surface, and into said interior core; and
manipulating said bioactive material-containing matrix along or around said longitudinal axis so as to shrink said interstices or pores to a second size and shape sufficient to restrict the migration of said bioactive material from said interior core across said exterior surface.
2. The method of claim 1 , further comprising: manipulating the constrictions to reduce a diameter of the exterior surface to reduce or preclude longitudinal migration of the bioactive material retained in the interior core of the surgical suture.
3. The method of claim 1 , wherein the interior core includes patterns configured to restrict flow of the bioactive materials along a length of the interior core.
4. The method of claim 3 , wherein the pattern includes at least one section with a diameter which is equal to a diameter of surgical suture, forming a bioactive material flow barrier.
5. The method of claim 1 , wherein compressing the matrix between an extended state and a compressed state alters the surgical suture along the longitudinal axis by a ratio greater than or equal to 2:1.
6. The method of claim 1 , wherein the first size of the interstices between interwoven filaments or the pores of the monofilament is equal to or greater than 20 microns in diameter.
7. The method of claim 1 , wherein when the matrix has been manipulated to shrink the interstices or pores to the second size, the filaments are about 22 degrees off of the longitudinal axis.