Methods of delivering a polypeptide molecule to Otx2 target cells using an Otx2 targeting peptide
The disclosure provides a method of delivering a polypeptide molecule to an Otx2 target cell, including contacting the target cell with a chimeric polypeptide having (i) a targeting peptide consisting of SEQ ID NO: 2 and (ii) the polypeptide molecule.
1. A method of delivering a polypeptide molecule to an Orthodenticle Homeobox 2 (Otx2) target cell selected from retinal ganglion neurons, retinal bipolar neurons and parvalbumin neurons, comprising
contacting the target cell with a chimeric polypeptide consisting of:
(i) a targeting peptide consisting of the amino acid sequence SEQ ID NO: 2 bonded with
(ii) a heterologous polypeptide molecule which is not an Otx2 protein, wherein the targeting peptide is located at the C-terminus of the chimeric polypeptide.
2. A method of delivering a polypeptide molecule to an Otx2 target cell selected from retinal ganglion neurons, retinal bipolar neurons and parvalbumin neurons in a subject comprising delivering a polypeptide molecule to the target cell according to the method of claim 1 , wherein the delivering comprises administering the polypeptide molecule to the subject by infusion.
3. A method of delivering a polypeptide molecule to an Orthodenticle Homeobox 2 (Otx2) target cell selected from retinal ganglion neurons, retinal bipolar neurons and parvalbumin neurons, comprising
contacting the target cell with a chimeric polypeptide consisting of:
(i) a targeting peptide consisting of the amino acid sequence of SEQ ID NO: 2 bonded with
(ii) a heterologous polypeptide molecule which is not an Otx2 protein, and
(iii) a VP16 trans-activator domain of herpes virus,
wherein the targeting peptide is located at the C-terminus of the chimeric polypeptide.
4. The method according to claim 3 , wherein the VP16 trans-activator domain contains the amino acid sequence of SEQ ID NO: 4.
5. A method of delivering a polypeptide molecule to an Otx2 target cell selected from retinal ganglion neurons, retinal bipolar neurons and parvalbumin neurons in a subject comprising delivering a polypeptide molecule to the target cell according to the method of claim 3 , wherein the delivering comprises administering the polypeptide molecule to the subject by infusion.