Bioactive peptide complexes
Herein disclosed are bioactive peptides and proteins having immunomodulating and antiviral activity, more particularly histidine-rich bioactive peptide complexes having the following structural formula (SEQ ID NO: 29): wherein: X 1 is preferably absent or is selected from among Gln, Ser, Asn, Val, Ala, Phe, and Asp, and R 1 and R 2 are peptide chains optionally contain the amino acid residues His and/or Cys that interact with transition metal ions. Whereas R 1 is preferably selected from among His-Gly-Val-Ser-Gly(SEQ ID NO: 30), CysVal-Val-Thr-Gly- (SEQ ID NO: 31), Cys-Gly-, Val-Ser-Gly-, and His-Gly- or alternatively is absent, R 2 is preferably selected from among -Val-His-Gly, -Val-Phe-Val, -Val-His, -Val-Asp or alternatively is absent. Such histidine-rich peptide complexes, primarily alloferon family peptides such as Alloferon-1 (SEQ ID NO: 1) with Zn ions, enable the creation of drugs with a targeted mechanism of action, and the design thereof with regard to understanding of drug target structure.
1. A peptide complex organized in three-dimensional structure and characterized by general structural formula:
wherein:
X 1 is selected from the group consisting of Gln, Ser, Asn, Val, Ala, Phe, and Asp or alternatively is absent; and
R 1 and R 2 and comprise peptide chains that interact with transition metal ions, further wherein R 1 is selected from the group consisting of His-Gly-Val-Ser-Gly- (SEQ ID NO: 30), Cys-Val-Val-Thr-Gly- (SEQ ID NO: 31), Cys-Gly-, Val-Ser-Gly-, and His-Gly- or alternatively is absent; and R 2 is selected from the group consisting of: -Val-His-Gly, -Val-Phe-Val, -Val-His, and -Val-Asp or alternatively is absent,
further wherein if X 1 is absent, R 2 must be present and if R 2 is absent, X 1 must be present.
2. The peptide complex according to claim 1 , wherein said peptide complex induces interferon synthesis.
3. The peptide complex according to claim 1 , wherein said peptide complex has antiviral activity.
4. The peptide complex according to claim 1 , wherein X 1 is present and is selected from the group consisting of Gln, Ser, Asn, Val, Ala, Phe, and Asp.
5. The peptide complex according to claim 1 , wherein R 2 is present and selected from the group consisting of: -Val-His-Gly, -Val-Phe-Val, -Val-His, and -Val-Asp.
6. The peptide complex according to claim 1 , wherein X 1 , R 1 , and R 2 are all present.
7. The peptide complex according to claim 1 , wherein the peptide component is 7 to 13 amino acid residues long.
8. The peptide complex according to claim 1 , wherein X 1 is Gln, R 1 is His-Gly-Val-Ser-Gly- (SEQ ID NO: 30), and R 2 is -Val-His-Gly.
9. A peptide complex organized in three-dimensional structure and characterized by general structural formula:
wherein:
X 1 is selected from the group consisting of Gln, Ser, Asn, Val, Ala, Phe, and Asp or alternatively is absent; and
R 1 and R 2 comprise peptide chains that interact with transition metal ions, further wherein R 1 is selected from the group consisting of: His-Gly-Val-Ser-Gly- (SEQ ID NO: 30), Cys-Val-Val-Thr-Gly- (SEQ ID NO: 31), Cys-Gly-, and Val-Ser-Gly-, or alternatively is absent; and R 2 is selected from the group consisting of: -Val-His-Gly, -Val-Phe-Val, -Val-His, and -Val-Asp or alternatively is absent.