Spinach plants that are resistant to downy mildew
The present invention relates to the field of spinach breeding, in particular to a new dominant resistance gene, designated RPF12, which confers resistance against all races of Peronospora farinosa and to spinach plants comprising said gene.
1. A spinach plant of the species Spinacia oleracea comprising resistance against Peronospora farinosa races 7-14, wherein said resistance is conferred by an introgression fragment comprising a single dominant gene, said introgression fragment comprising a Thymine for SNP_01 for nucleotide 101 of SEQ ID NO: 1, and a Thymine for SNP_02 for nucleotide 101 of SEQ ID NO: 2; wherein said gene is the gene present in plants grown from seeds, a representative sample of seeds having been deposited under accession number NCIMB 42159.
2. The plant according to claim 1 , wherein the gene further confers resistance against Peronospora farinosa races 1, 2, 3, 4, 5 and 6.
3. The plant according to claim 1 , wherein the gene further confers resistance against Peronospora farinosa race 15.
4. The spinach plant according to claim 1 , wherein the gene is located between SNP_01 at nucleotide 101 of SEQ ID NO: 1 and SNP_02 at nucleotide 101 of SEQ ID NO: 2.
5. The plant according to claim 1 , wherein said spinach plant is a hybrid plant.
6. The plant according to claim 1 , wherein said spinach plant is an inbred plant.
7. The plant according to claim 1 , wherein the spinach plant is savoy, semi-savoy, or smooth leaved.
8. The plant according to claim 1 , wherein said resistance against Peronospora farinosa is conferred by an introgression fragment from Spinacia turkestanica.
9. The plant according to claim 8 , wherein said introgression fragment is the fragment present in spinach seeds, a representative sample of seeds having been deposited under accession number NCIMB 42159, or a sub-fragment thereof.
10. A seed from which the plant according to claim 1 can be grown.
11. A leaf of the plant according claim 1 .
12. A progeny plant of the spinach plant according to claim 1 , wherein said progeny plant retains the introgression fragment comprising the resistance gene and comprising a Thymine for SNP_01 at nucleotide 101 of SEQ ID NO: 1, and a Thymine for SNP_02 for nucleotide 101 of SEQ ID NO: 2; which gene confers resistance to Peronospora farinosa races 7-14.
13. The progeny plant according to claim 12 , wherein said progeny plant is resistant against Peronospora farinosa races 7-14 and race 15.
14. The progeny plant according to claim 12 , wherein the progeny plant is produced by one or more of selfing, crossing, mutation, double haploid production and/or transformation.
15. A part of the spinach plant according to claim 1 , wherein the part is a stem, a cutting, a petiole, a cotyledon, a flower, an anther, a pollen, an ovary, a root, a root tip, a protoplast, a callus, a microspore, a stalk, an ovule, a shoot, a seed, an embryo, an embryo sac, a cell, a meristem, a bud or a leaf, and wherein said part comprises said introgression fragment.
16. A cell culture or tissue culture comprising cells or tissues derived from a part of a spinach plant of the species Spinacia oleracea comprising resistance against Peronospora farinosa races 7-14, wherein said resistance is conferred by an introgression fragment comprising a single dominant gene wherein said resistance gene is the gene present in plants grown from seeds, a representative sample of seeds having been deposited under accession number NCIMB 42159 and said introgression fragment comprises a Thymine for SNP_01 at nucleotide 101 of SEQ ID NO: 1, and a Thymine for SNP_02 at nucleotide 101 of SEQ ID NO: 2; and wherein said cells or tissues comprise said introgression fragment.
17. A spinach plant regenerated from the cell culture or tissue culture of claim 16 , and comprising resistance against Peronospora farinosa races 7-14 and comprising said introgression fragment.
18. A method for generating a spinach plant comprising resistance against Peronospora farinosa races 7-14 comprising:
a) crossing a first spinach plant of the species Spinacia oleracea that comprises resistance against Peronospora farinosa races 7-14 with a second spinach plant that is susceptible against one or more of Peronospora farinosa races 7 to 14,
wherein said resistance is conferred by an introgression fragment comprising a single dominant gene, and wherein said gene is the gene present in plants grown from seeds, a representative sample of seeds having been deposited under accession number NCIMB 42159 and wherein said introgression fragment comprises a Thymine for SNP_01 at nucleotide 101 of SEQ ID NO: 1, and a Thymine for SNP_02 at nucleotide 101 of SEQ ID NO: 2; and
b) growing a progeny plant from said crossing, wherein said progeny plant comprises said single gene.
19. The method of claim 18 , further comprising
c) selfing a plant grown from progeny of said crossing, one or more times to produce a further generation selfing progeny and/or backcrossing a plant grown from progeny of said crossing or grown from the further generation selfing progeny with a spinach plant is susceptible against one or more of Peronospora farinosa races 7 to 14; and
d) identifying a spinach plant among the progeny plants of step c) that comprises said single dominant gene of the first parent plant of step a).
20. A spinach plant obtained by the method of claim 19 , said spinach plant comprising resistance against Peronospora farinosa races 7-14 and comprising said introgression fragment.
21. The method according to claim 19 , wherein the introgression fragment further comprises a Thymine for SNP_02 at nucleotide 101 of SEQ ID NO: 2.
22. The method according to claim 19 , wherein the genotype of SNP_01 is used to identify a plant in step d).