Human artificial chromosome containing human antibody lambda light chain gene and non-human animal containing the human artificial chromosome capable of genetic transmission
The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
1. A transchromosomic mouse that carries a human artificial chromosome in which an SC20 chromosome vector is linked to an about 1 Mb to about 3.5 Mb fragment of human chromosome 22 comprising an unrearranged human antibody λ light chain locus.
2. The transchromosomic mouse of claim 1 , wherein the human artificial chromosome is ΔHAC.
3. The transchromosomic mouse of claim 1 , wherein the human artificial chromosome is ΔΔHAC.
4. The transchromosomic mouse of claim 1 , wherein the size of the fragment of human chromosome 22 comprising the unrearranged human antibody λ light chain locus is from about 1.2 Mb to about 3 Mb.
5. The transchromosomic mouse of claim 1 , wherein the size of the fragment of human chromosome 22 comprising the unrearranged human antibody λ light chain locus is from about 1.5 Mb to about 2.5 Mb.
6. The transchromosomic mouse of claim 1 , wherein the size of the fragment of human chromosome 22 comprising the unrearranged human antibody λ light chain locus is 1.5 Mb.
7. The transchromosomic mouse of claim 1 , wherein the size of the fragment of human chromosome 22 comprising the unrearranged human antibody λ light chain locus is 2.5 Mb.
8. The transchromosomic mouse of clam 1 , wherein the fragment of human chromosome 22 comprises the human λ light chain gene region HCF2-Igλ-AP000344.
9. The transchromosomic mouse of claim 1 , wherein the fragment of human chromosome 22 comprises the human λ light chain gene region AP000553-Igλ-AP000344.
10. The transchromosomic mouse of claim 1 , wherein at least both alleles of endogenous heavy chain locus and κ light chain locus are disrupted or inactivated.