Detergent composition
The present invention concerns a detergent and a pharmaceutical composition comprising a deoxyribonuclease (DNase), wherein the DNase is obtained from a fungal source. It further concerns a laundering method and the use of DNase. The present invention further relates to polypeptides having DNase activity, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides.
1. A method for preventing soil redeposition as an add-on effect to enzymes in a laundry detergent, comprising treating a textile soiled with bacteria with a polypeptide having DNase activity, the polypeptide from a fungal source, together with an enzyme that is not DNase, in a detergent, to produce a less-soiled textile, compared to treating the textile soiled with bacteria without the polypeptide having DNase activity, but with the enzyme that is not DNase in the detergent,
where the polypeptide from a fungal source has an amino acid sequence that includes SEQ ID NO: 2 or SEQ ID NO: 9.
2. The method of claim 1 , where the enzyme that is not a DNase includes a protease, amylase, lipase, mannase, pectate lyase or cellulase.
3. The method of claim 1 , where the textile soiled with bacteria is a dirty laundry item, and is treated with the polypeptide having DNase activity, together with the enzyme that is not DNase in the detergent, together with less dirty laundry items, to produce laundry items that are all less soiled after the treating than before the treating.
4. The method of claim 1 , where the textile soiled with bacteria includes textiles containing cotton, polyester, cotton/polyester, polyamide, polyacryl or silk.
5. A detergent composition comprising a polypeptide of SEQ ID NO: 2 or SEQ ID NO: 9 and a detergent adjunct ingredient.
6. The detergent composition of claim 5 , wherein the detergent adjunct ingredient is selected from the group consisting of surfactants, builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, builders and co-builders, fabric huing agents, anti-foaming agents, dispersants, processing aids, and pigments.
7. A method for laundering a textile comprising the steps of:
(a) exposing an item to a wash liquor comprising a detergent composition that includes a DNase from an Aspergillus ; and
(b) completing at least one wash cycle;
where the DNase from the Aspergillus has an amino acid sequence that includes SEQ ID NO: 2 or SEQ ID NO: 9.
8. The method of claim 7 , further comprising rinsing the textile.
9. A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide of SEQ ID NO: 2 or SEQ ID NO: 9, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in a recombinant host cell.
10. A recombinant host cell comprising the nucleic acid construct or expression vector of claim 9 .
11. A method of producing a polypeptide having DNase activity, comprising cultivating the recombinant host cell of claim 10 under conditions conducive for production of the polypeptide.