Immunochromatographic analysis method
An object is to provide an immunochromatographic analysis method capable of shortening the developing time without decreasing the detection sensitivity, and also capable of reducing the return of the liquid of a developed component, and a method for detecting a detection target contained in an analyte using an immunochromatographic analysis device including an absorption part composed of glass fiber, wherein the analyte and a labeling substance are developed in a chromatography medium part as a mobile phase in the presence of a nonionic surfactant, and the detection target is detected in a detection part is provided.
1. An immunochromatographic analysis method, which is a method for detecting a detection target contained in an analyte using an immunochromatographic analysis device which includes a sample addition part, a labeling substance retaining part, a chromatography medium part having a detection part supported thereon, and an absorption part that has a filtering time from 20 to 110 seconds and a density of 200 to 350 mg/cm 3 and is composed of glass fiber, comprising the following steps (1) to (4):
(1) a step of adding an analyte-containing solution obtained by diluting the analyte with an analyte dilution solution to the sample addition part;
(2) a step of allowing a labeling substance retained in the labeling substance retaining part to recognize the detection target;
(3) a step of allowing the analyte and the labeling substance in the chromatography medium part as a mobile phase in the presence of a nonionic surfactant to be developed; and
(4) a step of detecting the detection target in the developed mobile phase in the detection part.
2. The method according to claim 1 , wherein the nonionic surfactant is contained in at least one of the sample addition part and the analyte dilution solution.
3. The method according to claim 2 , wherein the content of the nonionic surfactant in the sample addition part is from 0.05 to 5 mg per cm 2 .
4. The method according to claim 2 , wherein the content of the nonionic surfactant in the analyte dilution solution is from 0.05 to 10 mass %.
5. The method according to claim 1 , wherein the hydrophilic-lipophilic balance value of the nonionic surfactant is 10 or more.
6. The method according to claim 1 , wherein the chromatography medium part contains an anionic surfactant.
7. The method according to claim 6 , wherein the content of the anionic surfactant in the chromatography medium part is from 0.02 to 4 mass %.
8. The method according to claim 1 , wherein the chromatography medium part is composed of nitrocellulose.
9. An immunochromatographic analysis device, sequentially comprising a sample addition part, a labeling substance retaining part, a chromatography medium part having a detection part supported thereon, and an absorption part, wherein the sample addition part retains a nonionic surfactant in a dry state, and the absorption part has a filtering time from 20 to 110 seconds and a density of 200 to 350 mg/cm 3 and is composed of glass fiber.
10. An immunochromatographic analysis kit, comprising an immunochromatography device, which sequentially includes a sample addition part, a labeling substance retaining part, a chromatography medium part having a detection part supported thereon and an absorption part, and an analyte dilution solution for diluting a detection target contained in an analyte, wherein the absorption part has a filtering time from 20 to 110 seconds and a density of 200 to 350 mg/cm 3 and is composed of glass fiber, and the analyte dilution solution contains a nonionic surfactant.
11. The method according to claim 2 , wherein the hydrophilic-lipophilic balance value of the nonionic surfactant is 10 or more.
12. The method according to claim 3 , wherein the hydrophilic-lipophilic balance value of the nonionic surfactant is 10 or more.
13. The method according to claim 4 , wherein the hydrophilic-lipophilic balance value of the nonionic surfactant is 10 or more.
14. The method according to claim 2 , wherein the chromatography medium part contains an anionic surfactant.
15. The method according to claim 3 , wherein the chromatography medium part contains an anionic surfactant.
16. The method according to claim 4 , wherein the chromatography medium part contains an anionic surfactant.
17. The method according to claim 5 , wherein the chromatography medium part contains an anionic surfactant.
18. The method according to claim 2 , wherein the chromatography medium part is composed of nitrocellulose.