Coryneform bacterium transformant and process for producing 4-hydroxybenzoic acid or salt thereof using the same
A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.
1. A transformant capable of producing 4-hydroxybenzoic acid or a salt thereof, the transformant being constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host,
wherein the gene which encodes an enzyme having chorismate-pyruvate lyase activity is the DNA of the following (a) or (b);
(a) a DNA consisting of the sequence of SEQ ID NO:2,
(b) a DNA which hybridizes to a DNA consisting of a complementary sequence of the DNA of (a) under stringent conditions or which consists of a sequence having 90% or more of homology with the sequence of (a), and which encodes a polypeptide having chorismate-pyruvate lyase activity.
2. The transformant of claim 1 , wherein the transformant is Corynebacterium glutamicum HBA-2 (Accession Number: NITE BP-01838).
3. A process for producing 4-hydroxybenzoic acid or a salt thereof, which comprises a step of culturing the transformant of claim 1 in a reaction mixture containing at least one starting compound selected from the group consisting of a sugar, a compound from which the transformant is capable of producing chorismic acid by metabolism, and chorismic acid or a salt thereof, and a step of recovering 4-hydroxybenzoic acid or a salt thereof from the reaction mixture.
4. The process of claim 3 , wherein the transformant is cultured under aerobic conditions where the transformant does not grow.