Immunoglobulins binding human Vγ9 Vδ2 T cell receptors
The invention is in the field of medicine and relates to immunology, and relates in particular to human Vγ9Vδ2 T cell receptor binding immunoglobulin molecules. Human Vγ9Vδ2 T cell receptor binding immunoglobulin molecules are in particular for use in medical treatment and/or useful in assays with human Vγ9Vδ2 T cells, wherein human Vγ9Vδ2 T cells may be modulated.
1. A human Vγ9Vδ2 T cell receptor binding immunoglobulin molecule, comprising a CDR1 region, and a CDR2 region,
and a CDR 3 region, wherein the CDR1, CDR2 and CDR3 regions comprise:
the amino acid sequences set forth in SEQ ID Nos: 4, 5 and 6, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 7, 8 and 9, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 4, 10 and 11, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 12, 13 and 14, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 15, 16 and 17, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 18, 19 and 20, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 18, 21 and 22, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 23, 24 and 25, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 15, 28 and 29, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 31, 32 and 33, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 31, 34 and 35, respectively, or
the amino acid sequences set forth in SEQ ID Nos: 44, 45 and 46, respectively.
2. The immunoglobulin molecule according to claim 1 , wherein the immunoglobulin molecule is a single chain antibody.
3. The immunoglobulin molecule according to claim 1 , wherein the immunoglobulin molecule is a single domain antibody.
4. The immunoglobulin molecule according to claim 1 , comprising one or more of the framework regions selected from the group of amino acid sequences of SEQ ID NOS. 67-70.
5. The immunoglobulin molecule according to claim 1 , wherein the immunoglobulin molecule has an amino acid sequence selected from the group of amino acid sequences consisting of SEQ ID NOS. 48-57, 59, 61, 62 and 66.
6. A nucleotide sequence that encodes an immunoglobulin molecule according to claim 1 .
7. A host cell comprising a nucleotide sequence according to claim 6 .
8. Method for preparing an immunoglobulin molecule according to claim 1 , comprising:
culturing a host cell comprising a nucleotide sequence that encodes the immunoglobulin molecule according to claim 1 ;
allowing the host cell to express the immunoglobulin;
obtaining the immunoglobulin.
9. An immunoglobulin molecule according to claim 1 , wherein the immunoglobulin molecule is an immunoglobulin molecule that activates human Vγ9Vδ2 T cells.
10. The immunoglobulin molecule according to claim 9 , wherein the immunoglobulin molecule is linked to an agent.
11. The immunoglobulin molecule according to claim 1 , which is a bispecific antibody comprising two single domain antibodies, the first single domain antibody comprising the CDR1, CDR2 and CDR3 regions as defined in claim 1 , wherein the first single domain antibody is linked to a second single domain antibody wherein the second single domain antibody binds to a target.
12. The immunoglobulin molecule according to claim 1 , which is a bispecific antibody comprising two single domain antibodies, the first single domain antibody comprising the CDR1, CDR2 and CDR3 regions as defined in claim 1 , wherein the first single domain antibody is linked to a second single domain antibody wherein the second single domain antibody binds to a cancer cell.
13. The immunoglobulin molecule of claim 2 , wherein the single chain antibody is a llama single chain antibody.
14. The immunoglobulin molecule of claim 2 , wherein the single chain antibody is a human single chain antibody.
15. The immunoglobulin molecule of claim 3 , wherein the single domain antibody is a llama single domain antibody.
16. The immunoglobulin molecule of claim 3 , wherein the single domain antibody is a human single domain antibody.