Recombinant host cell engineered to overexpress helper proteins
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
1. A method of manufacturing a heterologous protein of interest in an isolated host cell comprising:
a) providing an isolated host cell engineered to overexpress a polynucleotide encoding a helper protein having the amino acid sequence as shown in SEQ ID NO: 1 or a functional homologue thereof, wherein the amino acid sequence of the functional homologue has at least 95% sequence identity to the amino acid sequence as shown in SEQ ID NO: 1, wherein the isolated host cell comprises a heterologous polynucleotide encoding the heterologous protein of interest, wherein the heterologous protein of interest and the helper protein are different proteins, and wherein the yield of the heterologous protein of interest produced by the isolated host cell is increased as compared to a corresponding isolated host cell that does not overexpress the polynucleotide encoding the helper protein or the functional homologue;
b) culturing the isolated host cell under suitable conditions to overexpress the helper protein or the functional homologue and express the heterologous protein of interest; and
c) isolating the heterologous protein of interest from the cell culture.
2. The method of claim 1 , wherein the amino acid sequence of the functional homologue has at least 97% sequence identity to the amino acid sequence as shown in SEQ ID NO: 1.
3. The method of claim 1 , wherein the amino acid sequence of the functional homologue has at least 99% sequence identity to the amino acid sequence as shown in SEQ ID NO: 1.
4. The method of claim 1 , wherein the heterologous protein of interest comprises a mammalian polypeptide.
5. The method of claim 1 , wherein the heterologous protein of interest comprises a human polypeptide.
6. The method of claim 1 , wherein the heterologous protein of interest is an enzyme, a therapeutic protein, a food additive or a feed additive, or an antibody or an antibody fragment.