GM3 functionalized nanoparticles
Embodiments disclosed herein relates to ganglioside GM3-containing mixed lipids nanoparticles having an overall size between 60-100 nm, the making thereof and the uses. The nanoparticles selectively targeted to CD169+ expressing cells such as dendritic cells and macrophage. The nanoparticles are endocytosed by the CD169+ expressing cells.
1. A nanoparticle comprising:
a) a core having a largest diameter between 50-90 nm;
b) a coating layer encasing the core, wherein the core comprises a material selected from the group consisting of gold, silver, a gold alloy, a silver alloy, silica, mesoporous silica, polystyrene, and titania; and
c) a ganglioside GM3-containing mixed lipid layer comprising of dipalmitoylphosphatidylcholine (DPPC), cholesterol, phosphatidylserine (PS), and aNeu5Ac(2-3)bDGalp(1-4)bDG1cp(1-1)Cer (GM3); wherein the ganglioside GM3-mixed lipid layer is exterior of the coating layer and is integrated into the coating layer, and wherein the nanoparticles have an overall particle size between 60-100 nm.
2. The nanoparticle of claim 1 , wherein the core is a solid core.
3. The nanoparticle of claim 1 , wherein the core has a shape that is selected from the group consisting of ellipsoid, spherical, rod-like, octahedral, and cube-like.
4. The nanoparticle of claim 1 , wherein the coating layer is a thiolated lipid, a silane with long alkyl chains, or polyethylene glycol thiol.
5. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer consists of 40%-60% of DPPC.
6. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer consists of 40%-60% of cholesterol.
7. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer consists of 0.5%-5% of PS.
8. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer consists of 0.1%-6% of GM3.
9. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer optionally consist of galactosylceramide (Gal-Cer).
10. The nanoparticle of claim 1 , wherein the ganglioside GM3 containing mixed lipid layer consists of DPPC, cholesterol, PS, and GM3, and optionally Gal-Cer.
11. The nanoparticle of claim 1 , wherein the nanoparticle comprises a second layer of ganglioside GM3 containing mixed lipid.