Cell surface signature for processing cardiomyocyte subsets from heterogeneous cell samples
Methods are provided for distinguishing a cardiomyocyte subset in a heterogeneous cellular sample. Aspects of the methods include contacting the heterogeneous cellular sample with a cell surface marker specific binding member, and then distinguishing a cardiomyocyte subset of the cellular sample based on binding to the cell surface marker specific binding member. Also provided are devices, compositions and kits that find use in performing the subject methods.
1. A method of distinguishing a ventricular cardiomyocyte subset in a heterogeneous cellular sample, the method comprising:
contacting the heterogeneous cellular sample comprising ventricular cardiomyocytes, atrial cardiomyocytes, and non-cardiomyocytes with a ventricular cardiomyocyte cell surface marker specific binding member wherein the ventricular cardiomyocyte cell surface marker specific binding member is a CD77 specific binding member; and
distinguishing the ventricular cardiomyocyte subset based on whether the ventricular cardiomyocyte cell surface marker specific binding member binds to a cell surface marker on ventricular cardiomyocytes of the sample.
2. The method according to claim 1 , further comprising contacting the sample with one or more non-cardiomyocyte cell surface specific binding members.
3. The method according to claim 2 , wherein the one or more non-cardiomyocyte cell surface specific binding members are selected from the group consisting of specific binding members for CD90, CD140b, CD200, CD13, CD31, CD144, CD49a, CD167b and TRA-1-60.
4. The method according to claim 1 , further comprising contacting the sample with a cardiomyocyte cell surface specific binding member.
5. The method according to claim 4 , wherein the cardiomyocyte cell surface specific binding member is selected from the group consisting of specific binding members for CD172a, CD340, CD43 and CD29.
6. The method according to claim 1 , wherein the cell surface marker specific binding member is a non-cardiomyocyte cell surface specific binding member.
7. The method according to claim 6 , wherein the non-cardiomyocyte cell surface specific binding member is selected from the group consisting of specific binding members for CD90, CD140b, CD13, CD31, CD144, CD49a, CD167b, and TRA-1-60.
8. The method according to claim 1 , wherein distinguishing comprises at least one of separating, enriching, identifying and providing an assessment.
9. The method according to claim 1 , wherein one or more of the specific binding members comprises an antibody or a fragment thereof.
10. The method according to claim 1 , wherein the distinguishing comprises flow cytometry.
11. The method according to claim 1 , wherein the heterogeneous cell sample comprises cardiomyocyte precursors.
12. A composition comprising:
a heterogeneous cellular sample comprising live cardiomyocytes; and
a ventricular cardiomyocyte cell surface marker specific binding member, wherein the ventricular cardiomyocyte cell surface marker specific binding member is a CD77 specific binding member.
13. A kit comprising:
a ventricular cardiomyocyte cell surface marker specific binding member comprising a ventricular cardiomyocyte specific binding domain coupled to a first processing domain wherein the ventricular cardiomyocyte cell surface marker specific binding member is a CD77 specific binding member; and
a non-cardiomyocyte specific binding member comprising a non-cardiomyocyte specific binding domain coupled to a second processing domain.