Assay devices and method of detecting a target analyte
Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.
1. A assay device comprising:
a receptacle comprising a sample entry port;
a plunger disposed within the receptacle and having a first surface;
at least one reagent;
a membrane attached to the first surface of the plunger, the membrane comprising:
a target conjugate zone in which a plurality of affinity components are disposed;
a wick providing capillary force; and
a detection zone in which a plurality of capture compounds are immobilized,
wherein the detection zone is located between the target conjugate zone and the wick, wherein the plunger comprises a spring, wherein when the plunger is depressed, the spring engages with the membrane at the detection zone and the spring expands to detach the detection zone from being in fluid communication with the wick and to place the detection zone in fluid communication with the at least one reagent.
2. The assay device of claim 1 , wherein the at least one reagent is disposed in the receptacle.
3. The assay device of claim 1 , wherein the receptacle further comprises a barrier to separate the at least one reagent from the plunger.
4. The assay device of claim 1 , wherein the affinity components each comprise a binding portion comprising an antibody, a ligand, a peptide aptamer, a nucleotide aptamer, or a combination thereof.
5. The assay device of claim 1 , wherein the capture compounds comprise an aptamer, a peptide, an antibody, a ligand, or a combination thereof.
6. The assay device of claim 1 , wherein the plunger further comprises a second surface opposite the first surface and an end in communication with the first surface and the second surface, wherein the membrane is in contact with the end and with the second surface.
7. The assay device of claim 1 , wherein the receptacle comprises a test tube.