Systems and methods of preparing stabilized lipid assemblies
Direct polymerization of lipid monomers or polymer scaffolding of non-lipid monomers coupled with irradiation or redox polymerization performed at neutral pH resulted in stabilized lipid assemblies. An initiator-buffer component and NaHS03 redox mixture polymerizes reactive lipid monomers at near neutral pH conditions to preserve functionality of reconstituted membrane proteins. Improved stability of black lipid membranes (BLMs) is attained by chemical cross-linking of polymerizable, hydrophobic and commercially available non-lipid monomers partitioned into the suspended lipid membranes, and by suspending the BLMs across low surface energy apertures. Substrate apertures having low surface energy modifiers with amphiphobic properties facilitated a reproducible formation of BLMs by promoting interactions between the lipid tail and the substrate material. In addition, polymeric lipid bilayer membranes were prepared by photochemical or redox initiated polymerization of polymerizable lipid monomers, and disposed onto supporting substrates for use in chromatography columns.
1. A suspended lipid system comprising:
a. a supporting substrate having a substrate surface and an aperture;
b. a modified lipid membrane comprising a plurality of non-polymerizable lipid monomers and a plurality of hydrophobic non-lipid monomers dissolved in an organic solvent to form a solution, wherein said solution is disposed on the substrate such that the lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the non-lipid monomers are disposed in the suspended planar lipid bilayer and polymerized to stabilize the suspended planar lipid bilayer; and
c. one or more protein ion channels disposed through the suspended planar lipid bilayer.
2. The system of claim 1 , wherein the lipid bilayer is suspended across the aperture such that a first lipid leaflet and a second lipid leaflet of the lipid bilayer are both disposed above or below the aperture.
3. The system of claim 1 , wherein the lipid bilayer is suspended across the aperture such that a first lipid leaflet of the lipid bilayer is disposed above the aperture and a second lipid leaflet of the lipid bilayer is disposed below the aperture.
4. The system of claim 1 , wherein the supporting substrate is constructed from a material selected from a group consisting of a glass, a polymeric material, an epoxy, or a metal oxide.
5. The system of claim 1 , wherein an energy modifying layer is disposed on the substrate surface at or near the aperture, wherein the energy modifying layer is disposed between the lipid monomers and the substrate surface, wherein the energy modifying layer lowers a surface energy of the substrate surface.
6. The system of claim 5 , wherein the energy modifying layer lowers the surface energy of the supporting substrate to less than about 40 mJ/m 2 .
7. The system of claim 6 , wherein the energy modifying layer is a silane-modified layer, wherein the silane-modified layer comprises an alkylated silane.
8. The system of claim 7 , wherein the alkylated silane is selected from a group consisting of (tridecafluoro 1, 1, 2, 2-tetrahydrooctyl)-dimethylchlorosilane (PFDCS), (heptadecafluoro 1, 1, 2, 2-tetrahydrodecyl)-dimethylchlorosilane (PFDDCS) (tridecafluoro 1, 1, 2, 2-tetrahydrooctyl)-trichlorosilane (PFTCS), 3-cyanopropyldimethylchlorosilane (CPDCS), ethyldimethylchlorosilane (EDCS), aminopropyldimethylethoxyosilane (APDES), 3,3,3-trifluoropropyl-dimethylchlorosilane (FPDCS), or n-octyl-dimethylchlorosilane (ODCS).
9. The system of claim 1 , wherein the substrate surface has a surface energy of less than about 40 mJ/m 2 .
10. The system of claim 1 , wherein the non-lipid monomers comprise a methacrylate and a cross-linking agent.
11. The system of claim 10 , wherein the methacrylate is an aliphatic methacrylate or an aromatic methacrylate.
12. The system of claim 11 , wherein the aromatic methacrylate is a benzyl methacrylate or a naphthyl methacrylate.
13. The system of claim 10 , wherein the cross-linking agent is a dimethacrylate.
14. The system of claim 13 , wherein the dimethacrylate is ethylene glycol dimethacrylate.
15. The system of claim 1 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.
16. The system of claim 1 , wherein the lipid monomers are cell membrane fragments, 1,2-diphytanoyl-sn-glycero-3-phosphocholine monomers, naturally occurring lipids, or synthetic lipids.
17. The system of claim 1 , wherein the non-lipid monomers are polymerized by UV irradiation, visible irradiation, gamma irradiation, redox polymerization, or thermal polymerization.
18. The system of claim 17 , wherein the modified lipid membrane further comprises photoinitiators, wherein the non-lipid monomers are polymerized by UV or visible irradiation, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV.
19. The system of claim 18 , wherein the duration of UV or visible irradiation is sufficient to photopolymerize the non-lipid monomers.
20. The system of claim 17 , wherein the non-lipid monomers are polymerized by redox polymerization, wherein a redox polymerization mixture comprises an initiator-buffer component and NaHSO 3 .
21. The system of claim 20 , wherein the initiator-buffer component comprises ammonium persulfate, wherein a mole ratio of ammonium persulfate, NaHSO 3 , and lipid monomers is between about 10-500:10-500:1.
22. The system of claim 17 , wherein redox polymerization occurs at a near neutral pH.
23. The system of claim 17 , wherein the redox polymerization occurs at a pH between about 5 to 9.
24. A suspended lipid system comprising:
a. a supporting substrate having a substrate surface and an aperture, wherein an energy modifying layer is disposed on the substrate surface at or near the aperture, wherein the energy modifying layer lowers a surface energy of the substrate surface;
b. a modified lipid membrane comprising a plurality of non-polymerizable lipid monomers and a plurality of polymerizable, hydrophobic non-lipid monomers dissolved in an organic solvent to form a solution, the non-lipid monomers comprising a methacrylate and a cross-linking agent, wherein the solution is disposed on the substrate such that the lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the energy modifying layer is disposed between the lipid monomers and the substrate surface, wherein the non-lipid monomers are disposed in the suspended planar lipid bilayer and polymerized to stabilize the suspended planar lipid bilayer; and
c. one or more protein ion channels disposed through the suspended planar lipid bilayer;
wherein the plurality of polymerizable, hydrophobic non-lipid monomers are polymerized by redox polymerization using a redox polymerization mixture comprising an initiator-buffer component and NaHSO 3 , wherein redox polymerization occurs at a near neutral pH.
25. The system of claim 24 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.
26. A stabilized suspended lipid system comprising: a supporting substrate having a substrate aperture and a stabilized, planar lipid bilayer suspended across the aperture, wherein the stabilized, planar lipid bilayer has a polymer scaffold and protein ion channels disposed therein, wherein the stabilized, planar lipid bilayer is formed by:
a. preparing a monomer mixture comprising non-polymerizable lipid monomers and polymerizable, hydrophobic non-lipid monomers, dissolved in an organic solvent;
b. adding the monomer mixture to the substrate aperture, wherein the non-polymerizable lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the polymerizable, hydrophobic non-lipid monomers are disposed in the suspended planar lipid bilayer to form a modified lipid membrane;
c. polymerizing the non-lipid monomers to produce the polymer scaffold that stabilizes the modified lipid membrane; and
d. inserting one or more proteins through the lipid membrane to form the protein ion channels.
27. The system of claim 26 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.
28. A method of enhancing stability of a suspended lipid membrane, said method comprising:
a. providing a supporting substrate having a substrate surface and an aperture;
b. preparing a monomer mixture comprising non-polymerizable lipid monomers and polymerizable, hydrophobic non-lipid monomers, dissolved in an organic solvent;
c. adding the monomer mixture to the substrate aperture, wherein the non-polymerizable lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the polymerizable, hydrophobic non-lipid monomers are disposed in the suspended planar lipid bilayer to form a modified lipid membrane;
d. polymerizing the non-lipid monomers to stabilize the modified lipid membrane; and
e. inserting one or more proteins through the lipid membrane to form protein ion channels.
29. The method of claim 28 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 m, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.
30. A method of enhancing stability of a suspended lipid membrane, said method comprising:
a. depositing an energy modifying layer on a substrate surface at or near an aperture of a supporting substrate, wherein the energy modifying layer lowers a surface energy of the substrate surface;
b. preparing a monomer mixture comprising non-polymerizable lipid monomers and polymerizable, hydrophobic non-lipid monomers, dissolved in an organic solvent;
c. adding the monomer mixture to the substrate aperture, wherein the non-polymerizable lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the polymerizable, hydrophobic non-lipid monomers are disposed in the suspended planar lipid bilayer to form a modified lipid membrane;
d. polymerizing the non-lipid monomers to stabilize the modified lipid membrane; and
e. inserting one or more proteins through the lipid membrane to form protein ion channels.
31. The method of claim 30 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.
32. A method of enhancing stability of a suspended lipid membrane, said method comprising:
a. depositing an energy modifying layer on a substrate surface at or near an aperture of a supporting substrate, wherein the energy modifying layer lowers a surface energy of the substrate surface;
b. preparing a monomer mixture comprising non-polymerizable lipid monomers and polymerizable, hydrophobic non-lipid monomers, dissolved in an organic solvent, wherein the non-lipid monomers comprises a methacrylate and a cross-linking agent;
c. adding the monomer mixture to the substrate aperture, wherein the non-polymerizable lipid monomers form a planar lipid bilayer suspended across the aperture, wherein the energy modifying layer is disposed between the lipid monomers and the substrate surface, wherein the polymerizable, hydrophobic non-lipid monomers are disposed in the suspended planar lipid bilayer to form a modified lipid membrane;
d. polymerizing the non-lipid monomers using a redox polymerization mixture to stabilize the modified lipid membrane, wherein the redox polymerization mixtures comprises an initiator-buffer component and NaHSO 3 , wherein redox polymerization occurs at a near neutral pH; and
e. inserting one or more proteins through the lipid membrane to form protein ion channels.
33. The method of claim 32 , wherein a ratio of the non-polymerizable lipid monomers to the non-lipid monomers is 1:2, wherein a breakdown voltage of the modified lipid membrane is at least 1,250 mV, wherein an air-to-water transfer (AWT) count of the modified lipid membrane is at least 20, and wherein a lifetime of the modified lipid membrane is at least 24 hours.