Pharmaceutical compositions comprising CpG oligonucleotides
The present invention provides a pharmaceutical composition for inducing immune response in a subject, comprising: (a) an antigen with a concentration ranging from 1 μg/ml to 100 μg/ml; (b) CpG oligonucleotides having a sequence of 5′-tcgacgttcgtcgttcgtcgttc-3′, with a concentration ranging from 25 μg/ml to 500 μg/ml, and (c) an aluminum adjuvant with a concentration ranging from 25 μg/ml to 500 μg/ml. Such pharmaceutical composition can induce or boost immune response against antigen in the subject.
1. A pharmaceutical composition for inducing immune response to hepatitis C virus in a subject, comprising:
(a) a hepatitis C virus-related antigen with a concentration ranging from 1 μg/ml to 100 μg/ml;
(b) CpG oligonucleotides having a sequence of 5′-tcgacgttcgtcgttcgtcgttc-3′ (SEQ ID NO: 1), with a concentration ranging from 25 μg/ml to 500 μg/ml, and
(c) an aluminium adjuvant with a concentration ranging from 25 μg/ml to 500 μg/ml.
2. The pharmaceutical composition of claim 1 , wherein, the sequence of the said CpG oligonucleotides is 5′-tcgacgttcgtcgttcgtcgttc-3′ (SEQ ID NO: 1).
3. The pharmaceutical composition of claim 1 , wherein, the aluminium adjuvant comprises aluminium hydroxide.
4. The pharmaceutical composition of claim 1 , wherein, the aluminium adjuvant has a concentration ranging from 25 μg/ml to 125 μg/ml, or from 125 μg/ml to 500 μg/ml, or from 125 μg/ml to 400 μg/ml, or from 125 μg/ml to 300 μg/ml, or from 250 μg/ml to 500 μg/ml, or from 300 μg/ml to 400 μg/ml, or from 400 μg/ml to 500 μg/ml, or from 300 μg/ml to 500 μg/ml.
5. The pharmaceutical composition of claim 1 , wherein, the CpG oligonucleotides with the sequence of 5′-tcgacgttcgtcgttcgtcgttc-3′ (SEQ ID NO: 1) have a concentration ranging from 125 μg/ml to 500 μg/ml, or from 25 μg/ml to 125 μg/ml, or from 125 μg/ml to 250 μg/ml, or from 250 μg/ml to 500 μg/ml.
6. The pharmaceutical composition of claim 1 , wherein, the antigen has a concentration of 20 μg/ml.
7. The pharmaceutical composition of claim 1 , wherein,
the antigen has a concentration of 20 μg/ml, the CpG oligonucleotides have a concentration of 500 μg/ml, the aluminium adjuvant has a concentration of 25 μg/ml; or
the antigen has a concentration of 20 μg/ml, the aluminium adjuvant has a concentration of 25 μg/ml, the CpG oligonucleotides have a concentration of 125 μg/ml; or
the antigen has a concentration of 20 μg/ml, the aluminium adjuvant has a concentration of 125 μg/ml, the CpG oligonucleotides have a concentration of 25 μg/ml; or
the antigen has a concentration of 20 μg/ml, the aluminium adjuvant has a concentration of 300 μg/ml, the CpG oligonucleotides have a concentration of 125 μg/ml; or
the antigen has a concentration of 20 μg/ml, the aluminium adjuvant has a concentration of 400 μg/ml, the CpG oligonucleotides have a concentration of 125 μg/ml; or
the antigen has a concentration of 20 μg/ml, the aluminium adjuvant has a concentration of 125 μg/ml, the CpG oligonucleotides have a concentration of 500 μg/ml.
8. The pharmaceutical composition of claim 1 , further comprising pharmaceutically acceptable excipients.