Methods and compositions to modulate hair growth
Embodiments of the methods and compositions provided herein relate to inducing hair growth in a subject. Some embodiments include screening for agents to modulate hair growth.
1. A method for preparing a population of induced dermal papillae cells comprising:
(a) obtaining a population of human induced pluripotent stem cells (hIPSCs);
(b) differentiating the population of hIPSCs into a population of human induced pluripotent stem cell-derived neural crest cells (hIPSC-NC), wherein the population of hIPSC-NC lack a marker selected from the group consisting of versican, smooth muscle actin, and alkaline phosphatase; and
(c) differentiating the population of hIPSC-NC into a population of human induced pluripotent stem cell-derived dermal papillae-like cells (hIPSC-DP), wherein differentiating the population of hIPSC-NC comprises selecting adherent cells from the population of hIPSC-NC.
2. The method of claim 1 , wherein the population of human induced pluripotent stem cells are generated from a source selected from the group consisting of fibroblasts, renal epithelial cells, and blood cells.
3. The method of claim 1 , wherein the population of human induced pluripotent stem cells are generated from human BJ fibroblasts.
4. The method of claim 1 , wherein step (b) further comprises culturing the population of human induced pluripotent stem cells under conditions to form clusters.
5. The method of claim 4 , wherein the clusters are in suspension to form spheres.
6. The method of claim 5 , wherein the spheres are plated on the surface of a substrate, and wherein the surface is coated with fibronectin or polyornithine.
7. The method of claim 1 , wherein step (b) further comprises analyzing the population of hIPSC-NC to confirm the presence of a marker selected from the group consisting of Sox10, Foxd3, integrin alpha 4, cognate receptor for fibronectin, CD47, CD 184, CD44, P-75, and nestin.
8. The method of claim 1 , wherein step (b) further comprises analyzing the hIPSC-NC to confirm the absence of a marker selected from the group consisting of OCT4 and SSEA4.
9. The method of claim 1 , wherein step (c) further comprises analyzing the hIPSC-DP to confirm the presence of a marker selected from the group consisting of P-75, nestin, versican, smooth muscle actin, alkaline phosphatase, and vimentin.
10. The method of claim 1 , further comprising:
(d) administering the hIPSC-DP to a subject in need thereof.
11. The method of claim 10 , wherein the hIPSCs are obtained from the subject in need thereof.
12. The method of claim 10 , wherein the hIPSCs are not obtained from the subject in need thereof.
13. The method of claim 10 , wherein the administering comprises subcutaneous transplantation.
14. The method of claim 10 , further comprising administering to a location comprising hair on a skin of the subject in need thereof.
15. The method of claim 14 , wherein the location is selected from the group consisting of scalp, face, upper lip, chin, eyebrow, eyelash, arm, leg, back, torso, and abdomen.
16. The method of claim 14 , wherein the location comprises scar tissue.
17. The method of claim 10 , wherein the subject has alopecia.