System and method for assessing embryo viability
Disclosed is a system for determining fecundity of an embryo utilizing a non¬ invasive grading of early stage embryos (pre-hatching) based upon specific gravity, density and/or estimated weight. The system allows 100% recovery of embryos and can detect differences in growth potential at the earliest stages of development. The system may further enhance the development of embryos by utilization of microfluidic effects during use. The disclosed system supports a wide variety of scenarios for human and animal reproductive technologies and related products and services.
1. A method of non-invasive selection and recovery of viable mammalian embryos having enhanced embryo development, comprising:
descending a plurality of embryos through a drop chamber comprising a lumen containing a biocompatible media composition extending vertically from a recovery pool having fluid communication with the lumen of the drop chamber, wherein the recovery pool is capable of receiving the embryos from the lumen of the drop chamber, allowing for embryo recovery;
measuring the plurality of embryos while descending through a predetermined timing zone of said drop chamber having an upper and lower boundary of said predetermined timing zone, wherein said measuring step comprises:
a. detecting the movement downward of each embryo through the biocompatible media composition in the drop chamber, and
b. identifying descent time of each embryo moving downward through the biocompatible media composition between the upper and lower boundaries of said predetermined timing zone to determine the estimated weight of each embryo, wherein a faster descent time indicates a higher estimated weight,
selecting at least one of the plurality of embryos having a higher estimated weight; and
recovering said at least one embryo selected from the recovery pool, wherein the at least one embryo recovered has enhanced viability and embryo development.
2. The method of claim 1 , wherein said measuring step further comprises:
c. determining specific gravity of the plurality of embryos by measuring the descent time of each embryo through the biocompatible media composition within the upper and lower boundary of said predetermined timing zone of the drop chamber capable of detection by a means selected from the group consisting of: visual means, tagging of the embryos, markers detected on or in the embryos, computerized means of embryo recognition utilizing a processor having programmable logic designed to detect each embryo during descent through the timing zone, or combinations thereof.
3. The method of claim 1 , further comprising comparing the identified descent time of each embryo in measuring step b to the mean or median descent times of a plurality of embryos of the same species which were previously measured, wherein a faster descent time further indicates a higher specific gravity and the at least one embryo selected further has a higher specific gravity than the mean or median of the plurality of embryos.
4. The method of claim 1 , wherein the plurality of embryos range from zygote to blastocyst stage.
5. The method of claim 4 , further comprising comparing the identified descent time of each embryo ranging from zygote to blastocyst stage in measuring step b within a population mean wherein a faster descent time indicates a higher estimated weight.
6. The method of claim 1 , further comprising utilizing one or more drop chambers, for assessing one or more embryos, each of the one or more drop chambers emptying into one or more recovery pools to allow selection and recovery of one or more embryos having separate identifications.
7. The method of claim 1 , wherein said plurality of embryos comprises cryopreserved embryos, and the at least one embryo selected will survive after thaw.
8. The method of claim 1 , wherein said measuring step performed by descending the embryos through the biocompatible media composition of the drop chamber does not reduce the viability of the plurality of embryos.